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Microbial elements mining and systematic metabolic engineering




Principal Investigator:WANG Weishan, Ph.D., Professor

Add.:Room H611,NO.1 West Beichen Road, Chaoyang District, Beijing 100101, China

Research interests

Our lab works on the metabolic engineering and synthetic biological research in Streptomyces species and other model micro-organisms, and aims to construct intelligent and synthetic cell factories for high value-added products:
1. Developing approaches to identify the regulatory network and to mine the useful genetic elements or parts for synthetic biological application;
2. Exploiting engineering strategies for titer improvement of desired products;
3. Developing high-throughput methods for strain screening in evolutionary engineering.





2013 Ph.D., Biochemistry and Molecular Biology, University of Chinese Academy of Sciences, Beijing, China
2009 Master of Medicine, Ocean University of China, Qingdao, China
2006 Bachelor of Science, Shandong Agricultural University, Tai’an, China

Research experiences


Professor/Principle Investigator, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Associate Professor, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Assistant professor, Institute for Microbiology, Chinese Academy of Sciences, Beijing, China



Selected publications


1.   Li S, Yang B, Tan G-Y, Ouyang L-M, Qiu S, Wang W*, Xiang W*, Zhang L*: Polyketide pesticides from actinomycetes. Current Opinion in Biotechnology 2021, 69:299-307.

2.    Zhao X.#, Li S.#, Liu G.#, Wang Z.#, Yang Z., Zhang Q., Liang M., Liu J., Li Z., Tong Y., Zhu G., Wang X., Jiang L., Wang W.*, Tan G-Y*, Zhang L.*: A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes. Sci Bull 2021, 66:69-77

3.          Li S., Li Z., Xiang W., Pang S., Wang W.*: Coordinating precursor supply for pharmaceutical polyketide production in Streptomyces. Curr Opin Biotechnol 2020, 69:26-34.

4.          Wang W#,*,, Li S#, Li Z#, Zhang J#, Fan K, Tan G, Ai G, Lam SM, Shui G, Yang Z, Lu H, Jin P, Li Y, Chen X, Xia X, Liu X, Dannelly HK, Yang C, Yang Y, Zhang S, Alterovitz G, Xiang W*, Zhang L*: Harnessing the intracellular triacylglycerols for titer improvement of polyketides in Streptomyces. Nat Biotechnol 2020. 38(1):76-83.

5.          Yang Z#, Sun Q#, Tan G, Zhang Q, Wang Z, Li C, Qi F, Wang W*, Zhang L*, Li Z*: Engineering thermophilic Geobacillus thermoglucosidasius for riboflavin production. Microbial Biotechnology 2020, n/a(n/a). doi10.1111/1751-7915.13543

6.          Wang W#,*,, Li S#, Li Z#, Zhang J#, Fan K, Tan G, Ai G, Lam SM, Shui G, Yang Z, Lu H, Jin P, Li Y, Chen X, Xia X, Liu X, Dannelly HK, Yang C, Yang Y, Zhang S, Alterovitz G, Xiang W, Zhang L: Harnessing the intracellular triacylglycerols for titer improvement of polyketides in StreptomycesNat Biotechnol 2019. (DOI: 10.1038/s41587-019-0335-4)

7.          Wang X#, Yin S#, Bai J, Liu Y, Fan K, Wang H, Yuan F, Zhao B*, Li Z*, Wang W*: Heterologous production of chlortetracycline in an industrial grade Streptomyces rimosus host. Appl Microbiol Biotechnol 2019, 103(16):6645-6655.

8.          Liang M#Li Z#Wang W#, Liu J#, Liu L, Zhu G, Karthik L, Wang M, Wang KF, Wang Z, Yu J, Shuai Y, Yu J, Zhang L, Yang Z, Li C, Zhang Q, Shi T, Zhou L, Xie F, Dai H, Liu X, Zhang J, Liu G, Zhuo Y, Zhang B, Liu C, Li S, Xia X, Tong Y, Liu Y, Alterovitz G, Tan G*, Zhang L*: A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules. Nat Commun 2019, 10(1):3672.

9.          Wang J#Wang W, Wang H, Yuan F, Xu Z, Yang K, Li Z*, Chen Y*, Fan K*: Improvement of stress tolerance and riboflavin production of Bacillus subtilis by introduction of heat shock proteins from thermophilic bacillus strains. Appl Microbiol Biotechnol 2019, 103(11):4455-4465.

10.       Cao J#, Yao Y#, Fan K, Tan G, Xiang W, Xia X, Li S*, Wang W*, Zhang L*: Harnessing a previously unidentified capability of bacterial allosteric transcription factors for sensing diverse small molecules in vitroSci Adv 2018, 4(11):eaau4602.

11.       Yao Y#, Li S#, Cao J, Liu W, Fan K, Xiang W, Yang K*, Kong D*, Wang W*: Development of small molecule biosensors by coupling the recognition of the bacterial allosteric transcription factor with isothermal strand displacement amplification. Chem Commun (Camb) 2018, 54(38):4774-4777.

12.       Yao Y#, Li S#, Cao J, Liu W, Qi F, Xiang W, Yang K, Wang W*, Zhang L*: A novel signal transduction system for development of uric acid biosensors. Appl Microbiol Biotechnol 2018, 102(17):7489-7497.

13.       Li S#, Wang J#, Xiang W, Yang K, Li Z*, Wang W*: An Autoregulated Fine-Tuning Strategy for Titer Improvement of Secondary Metabolites Using Native Promoters in StreptomycesACS Synth Biol 2018, 7(2):522-530.

14.       Li X#, Wang J, Shi M, Wang W, Corre C, Yang K*: Evidence for the formation of ScbR/ScbR2 heterodimers and identification of one of the regulatory targets in Streptomyces coelicolorAppl Microbiol Biotechnol 2017, 101(13):5333-5340.

15.       Li S#, Zhou L, Yao Y, Fan K, Li Z, Zhang L, Wang W*, Yang K*: A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays. Chem Commun (Camb) 2017, 53(1):99-102.

16.       Wang W#, Yang T#, Li Y, Li S, Yin S, Styles K, Corre C, Yang K*: Development of a Synthetic Oxytetracycline-Inducible Expression System for Streptomycetes Using de Novo Characterized Genetic Parts. ACS Synth Biol 2016, 5(7):765-773.

17.       Yin S#, Li Z#, Wang X, Wang H, Jia X, Ai G, Bai Z, Shi M, Yuan F, Liu T, Wang W*, Yang K*: Heterologous expression of oxytetracycline biosynthetic gene cluster in Streptomyces venezuelae WVR2006 to improve production level and to alter fermentation process. Appl Microbiol Biotechnol 2016, 100(24):10563-10572.

18.       Jiang T#, Ke B, Chen H, Wang W, Du L, Yang K, Li M*: Bioluminescent Probe for Detecting Mercury(II) in Living Mice. Anal Chem 2016, 88(15):7462-7465.

19.       Sidda JD#, Poon V, Song L, Wang W, Yang K, Corre C*: Overproduction and identification of butyrolactones SCB1-8 in the antibiotic production superhost Streptomyces M1152. Org Biomol Chem 2016, 14(27):6390-6393.

20.       Jiang T#Wang W, Wu X, Wu W, Bai H, Ma Z, Shen Y, Yang K*, Li M*: Discovery of New Substrates for LuxAB Bacterial Bioluminescence. Chem Biol Drug Des 2016, 88(2):197-208.

21.       Li S#, Wang J#, Li X, Yin S, Wang W*, Yang K*: Genome-wide identification and evaluation of constitutive promoters in streptomycetes. Microb Cell Fact 2015, 14(1):172.

22.       Li S#Wang W#, Li X, Fan K, Yang K*: Genome-wide identification and characterization of reference genes with different transcript abundances for Streptomyces coelicolor. Sci Rep 2015, 5:15840.

23.       Li X#, Wang J, Li S, Ji J, Wang W*, Yang K*: ScbR- and ScbR2-mediated signal transduction networks coordinate complex physiological responses in Streptomyces coelicolor. Sci Rep 2015, 5:14831.

24.       Wang W#, Li X#, Li Y, Li S, Fan K, Yang K*: A genetic biosensor for identification of transcriptional repressors of target promoters. Sci Rep 2015, 5:15887.

25.       Yin S#Wang W#, Wang X, Zhu Y, Jia X, Li S, Yuan F, Zhang Y*, Yang K*: Identification of a cluster-situated activator of oxytetracycline biosynthesis and manipulation of its expression for improved oxytetracycline production in Streptomyces rimosusMicrob Cell Fact 2015, 14(1):46.

26.       Wang W#, Ji J#, Li X, Wang J, Li S, Pan G, Fan K, Yang K*: Angucyclines as signals modulate the behaviors ofStreptomyces coelicolorProc Natl Acad Sci U S A 2014, 111(15):5688-5693.

27.       Wang W#, Li X#, Wang J, Xiang S, Feng X, Yang K*: An engineered strong promoter for streptomycetes. Appl Environ Microbiol 2013, 79(14):4484-4492.

28.       Liu Z#Wang W, Zhu Y, Gong Q, Yu W*, Lu X*: Antibiotics at subinhibitory concentrations improve the quorum sensing behavior of Chromobacterium violaceumFEMS Microbiol Lett 2013, 341(1):37-44.

29.       Fan K#, Pan G#, Peng X, Zheng J, Gao W, Wang J, Wang W, Li Y, Yang K*: Identification of JadG as the B ring opening oxygenase catalyzing the oxidative C-C bond cleavage reaction in jadomycin biosynthesis. Chem Biol 2012, 19(11):1381-1390.

30.       Wang J#Wang W#, Wang L, Zhang G, Fan K, Tan H, Yang K*: A novel role of 'pseudo'gamma-butyrolactone receptors in controlling gamma-butyrolactone biosynthesis in StreptomycesMol Microbiol 2011, 82(1):236-250.


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Institute Of Microbiology Chinese Academy of Sciences
NO.1 West Beichen Road, Chaoyang District, Beijing 100101, China Phone: 0086-10-64807462 Fax: 0086-10-64807468 Email: office@im.ac.cn