Molecular Mechanism of RNA Silence and Disease Resistance in Plant----INSTITUTE OF MICROBOLOGY CHINESE ACADEMY OF SCIENCES

Principal Investigator：GUO Huishan, Ph.D., Professor

State Key Laboratory of Plant Genomics

Telephone/Fax：+86 (0) 10-64847989
E-mail：guohs@im.ac.cn
Add.:NO.1 West Beichen Road, Chaoyang District, Beijing 100101, China

Research interests

Her research group mainly focuses on RNA silencing and sRNA regulated pathways during plant-microbe interactions, for the purpose of dissecting mechanisms of plant resistance and susceptibility to virus well as fungi. The ongoing projects mainly use Arabidopsis and Nicotiana as model plants for study but all the results aim to apply for major crops such as cotton and potato.

Education

1983.9-1987.7

BSc Sun Yat-sen (Zhongshan) University, China

1993.6-1996.6

PhD Centro Nacional de Biotecnologia CSIC, Universidad Autonoma de Madrid, Spain

Employment & Professional Experience

1987.9-1991.12

Teaching Assistant, Center of Biotechnology, Zhongshan University, China

1992.1-1993.5

Guest Investigator,Centro de Biologia Molecular "Severo Ochoa", Universidad Autonoma de Madrid, Spain

1997.7-1998.6

Postdoctoral Fellow, Centro Nacional de Biotecnologia CSIC. Universidad Autonoma de Madrid, Spain

1998.7-2001.6

Research Fellow, Institute of Molecular Agrobiology, National University of Singapore

2001.7-2003.1

Research Fellow, Temasek Life Sciences Laboratory, National University of Singapore

2003.1-2004.6

Acting Principal Investigator, Temasek Life Sciences Laboratory, Singapore

2004.7-present

Principle Investigator, the “Hundred Talents” Program of Chinese Academy of Sciences, Institute of Microbiology, CAS, China

Honors andawards

2005

Received “Outstanding Youth Scientist Award (National Science Foundation of China)”

2015

SpecialgovernmentallowancesoftheStateCouncil

Special Professor of University of Chinese Academy of Sciences

Special Key Talents of Chinese Academy of Sciences

2017

Special Core Talents of Chinese Academy of Sciences

Academic Participation
Member of the Academic Committee of IMCAS
Deputy Director of the 4^thAcademic Committee of State Key laboratory of Plant Genomics
Member of the 8^thAcademic Committee of IMCAS (2008-2012)
Member ofAcademic ethics and equity committeeof IMCAS (2008-2012)
Member of the3^thAcademic Committeeof State Key laboratory of Plant Genomics(2011-2015)
Member of the 9^thCouncil ofChinese Society of Plant Pathology (2010-2014)
Member of the 1^thAcademic Committee ofKey Laboratory of Tropical Crops Germplasm Resources Utilization (2011-2015)
MPP Editorial Board 2018-

PBJ Editorial Board 2018-

PloS Pathogens Associate Editor 2014-

PloS Pathogens Guest Editor 2013-2014

BMC Genomics Associate Editor 2014-2018

MPP Associate Editor 2012-2017

MPMI Associate Editor 2013-2015

Selected publications  and Books（* corresponding author）:
^{1.      Cui, C.#, Wang, J.J.#, Zhao, J.H.#, Fang, Y.Y, He, X.F.,Guo, H.S.*,Duan, C.G.*(2020). A Brassica miRNA Regulates Plant Growth and Immunity through Distinct Modes of Action.Molecular Plant13(2): 231-245.
2.      Gao, F.#, Zhang, B.S.#, Zhao, J.H., Huang, J.F., Jia, P.S., Wang, S., Zhang, J., Zhou, J.M.,Guo, H.S.*（2019）Deacetylation of chitin oligomers increases virulence in soil-borne fungal pathogens.Nature Plants5(11): 1167-1176.
3.      Zhao, J. H.,Guo, H.S.*（2019）.Trans-kingdom RNA interactions drive the evolutionary arms race between hosts and pathogens.Curr Opin Genet Dev58-59:62-69.
4.      Liu, X.L., Zhao, J.H.,Guo, H.S.*（2019）.IBM1-dependent H3K9 demethylation enables self-silencing of an exogenous silencer for the non-cell autonomous silencing of an endogenous target gene.J Genet Genomics46（3）：149-153.
5.      Jin, Y., Zhao, J. H., Zhao, P., Zhang, T., Wang, S.,Guo, H.S.*.（2019）A fungal milRNA mediates epigenetic repression of a virulence gene in Verticillium dahliae.Philos Trans R Soc Lond B Biol Sci374（1767）：20180309.
6.      Zhao, J. H., Liu, X. L., Fang, Y. Y., Fang, R. X.,Guo, H.S.*.（2018）CMV2b-Dependent Regulation of Host Defense Pathways in the Context of Viral Infection.Viruses10（11).
7.      Qin, J., Wang, K., Sun, L., Xing, H., Wang, S., Li, L., Chen, S.,Guo, H.S.*, and Zhang, J.* (2018). The plant specific transcription factors CBP60g and SARD1 are targeted by a Verticillium secretory protein VdSCP41 to modulate immunity.eLife7.
8.      Jin, Y.^#, Zhao, P.^#, Fang, Y.Y., Gao, F.,Guo, H.S.*, and Zhao, J.H.* (2018). Genome-wide profiling of sRNAs in the Verticillium dahliae-infected Arabidopsis roots.Mycology, 1-11.
9.      Hua,C.L.#, Zhao, J.H.#, andGuo, H.S.*(2018).Trans-kingdom RNA silencing in Plant–Fungal Pathogen Interactions.Mol Plant11,235-244.
10.    Du, X., Wang, S., Gao, F., Zhang, L., Zhao, J.H.,Guo, H.S.*, and Hua, C.L.* (2017). Expression of pathogenesis-related genes in cotton roots in response to Verticillium dahliae PAMP molecules.Science China. Life sciences60, 852-860.
11.    Jin, Y., Zhang, T., andGuo, H.S.*(2017). Application of host induced gene silencing in crop protection against fungal diseases.Sheng wu gong cheng xue bao = Chinese journal of biotechnology33, 161-169.
12.    Zhang, L.S.#, Ni, H.#, Du, X., Wang, S., Ma, X.W., Nurnberger, T.,Guo, H.S.*, and Hua, C.L.* (2017). The Verticillium-specific protein VdSCP7 localizes to the plant nucleus and modulates immunity to fungal infections.The New phytologist215, 368-381.
13.    Zhao, Y.L.*, Zhang, T., andGuo, H.S. (2017). Penetration Assays, Fungal Recovery and Pathogenicity Assays for Verticillium dahliae.Bio-Protocol7.
14.    Zhou, T.T., Zhao, Y.L., andGuo, H.S.*(2017). Secretory proteins are delivered to the septin-organized penetration interface during root infection by Verticillium dahliae.PLoS pathogens13, e1006275.
15.    Fang, Y.Y.#, Zhao, J.H.#, Liu, S.W., Wang, S., Duan, C.G., andGuo, H.S.*(2016). CMV2b-AGO Interaction Is Required for the Suppression of RDR-Dependent Antiviral Silencing in Arabidopsis.Frontiers in Microbiology7, 1329.
16.    Lv, D.Q.#, Liu, S.W.#, Zhao, J.H., Zhou, B.J., Wang, S.P.,Guo, H.S., and Fang, Y.Y. * (2016). Replication of a pathogenic non-coding RNA increases DNA methylation in plants associated with a bromodomain-containing viroid-binding protein.Scientific reports6, 35751.
17.    Wang, S., Xing, H., Hua, C.,Guo, H.S.*, and Zhang, J.* (2016). An improved single-step cloning strategy simplifies the Agrobacterium tumefaciens-mediated transformation (ATMT)-based gene disruption method in Verticillium dahliae.Phytopathology.
18.    Zhang, T., Jin, Y., Zhao, J.H., Gao, F., Zhou, B.J., Fang, Y.Y., andGuo, H.S.*(2016). Host-Induced Gene Silencing of the Target Gene in Fungal Cells Confers Effective Resistance to the Cotton Wilt Disease Pathogen Verticillium dahliae.Mol Plant9, 939-942.
19.    Zhang, T.#, Zhao, Y.L.#, Zhao, J.H.#, Wang, S., Jin, Y., Chen, Z.Q., Fang, Y.Y., Hua, C.L., Ding, S.W., andGuo, H.S.*(2016). Cotton plants export microRNAs to inhibit virulence gene expression in a fungal pathogen.Nature plants2, 16153.
20.    Zhao, J.H.*, Fang, Y.Y., Duan, C.G., Fang, R.X., Ding, S.W., andGuo, H.S.*(2016). Genome-wide identification of endogenous RNA-directed DNA methylation loci associated with abundant 21-nucleotide siRNAs in Arabidopsis.Scientific reports6, 36247.
21.    Zhao, J.H., Hua, C.L., Fang, Y.Y., andGuo, H.S.*(2016). The dual edge of RNA silencing suppressors in the virus-host interactions.Current opinion in virology17, 39-44.
22.    Zhao, Y.L., Zhou, T.T., andGuo, H.S.*(2016). Hyphopodium-Specific VdNoxB/VdPls1-Dependent ROS-Ca²⁺Signaling Is Required for Plant Infection by Verticillium dahliae.PLoS pathogens12, e1005793.
23.    Fang, Y.Y., Smith, N.A., Zhao, J.H., Lee, J.R.,Guo, H.S., and Wang, M.B.* (2015). Cloning and profiling of small RNAs from cucumber mosaic virus satellite RNA.Methods in molecular biology1236, 99-109.
24.    Jin, Y., andGuo, H.S.*(2015). Transgene-induced gene silencing in plants. Methods Mol Biol105-117.
25.    Shen, W.X., Au, P.C., Shi, B.J., Smith, N.A., Dennis, E.S.,Guo, H.S., Zhou, C.Y., and Wang, M.B.* (2015). Satellite RNAs interfere with the function of viral RNA silencing suppressors.Front Plant Sci281.
26.    Wang, J.J., Zhang, L., andGuo, H.S.*(2015). Arabidopsis Leaf Explant Culture.Bio-Protocol5(22).
27.    Wang, J.J., andGuo, H.S.*(2015). Cleavage of INDOLE-3-ACETIC ACID INDUCIBLE28 mRNA by microRNA847 upregulates auxin signaling to modulate cell proliferation and lateral organ growth in Arabidopsis.The Plant Cell574-590.
28.    Zahid, K.#, Zhao, J.H.#, Smith, N.A., Schumann, U., Fang, Y.Y., Dennis, E.S., Zhang, R.,Guo, H.S.*, and Wang, M.B.* (2015). Nicotiana small RNA sequences support a host genome origin of cucumber mosaic virus satellite RNA.PLoS genetics11, e1004906.
29.    Zhao, P., Zhao, Y.L., Jin, Y., Zhang, T., andGuo, H.S.* (2014). Colonization process of Arabidopsis thaliana roots by a green fluorescent protein-tagged isolate of Verticillium dahliae.Protein & cell5, 94-98.
30.    Feng, L.#, Duan, C.G.#, andGuo, H.S.*(2013). Inhibition of in vivo Slicer activity of Argonaute protein 1 by the viral 2b protein independent of its dsRNA-binding function.Molecular plant pathology14, 617-622.
31.    Yang, L.P., Fang, Y.Y., An, C.P., Dong, L., Zhang, Z.H., Chen, H., Xie, Q., andGuo, H.S.*(2013). C2-mediated decrease in DNA methylation, accumulation of siRNAs, and increase in expression for genes involved in defense pathways in plants infected with beet severe curly top virus.The Plant journal73, 910-917.
32.    Duan, C.G.#, Fang, Y.Y.#, Zhou, B.J., Zhao, J.H., Hou, W.N., Zhu, H., Ding, S.W., andGuo, H.S.*(2012). Suppression of Arabidopsis ARGONAUTE1-mediated slicing, transgene-induced RNA silencing, and DNA methylation by distinct domains of the Cucumber mosaic virus 2b protein.The Plant cell24, 259-274.
33.    Duan, C.G., Wang, C.H., andGuo, H.S.*(2012). Application of RNA silencing to plant disease resistance.Silence3, 5.
34.    Xu, N., Li, Y., Zhao, Y.T., Guo, L., Fang, Y.Y., Zhao, J.H., Wang, X.J., Huang, L., andGuo, H.S.*(2012). Identification and characterization of small RNAs in the hyperthermophilic archaeon Sulfolobus solfataricus.PloS one7, e35306.
35.    Zhou, B.J., Jia, P.S., Gao, F., andGuo, H.S.*(2012). Molecular characterization and functional analysis of a necrosis- and ethylene-inducing, protein-encoding gene family from Verticillium dahliae.Molecular plant-microbe interactions : MPMI25, 964-975.
36.    Zhu, H., andGuo, H.S.*(2012). The role of virus-derived small interfering RNAs in RNA silencing in plants.Science China. Life sciences55, 119-125.
37.    Dong, L., Liu, M., Fang, Y.Y., Zhao, J.H., He, X.F., Ying, X.B., Zhang, Y.Y., Xie, Q., Chua, N.H., andGuo, H.S.*(2011). DRD1-Pol V-dependent self-silencing of an exogenous silencer restricts the non-cell autonomous silencing of an endogenous target gene.The Plant journal68, 633-645.
38.    Hou, W.N., Duan, C.G., Fang, R.X., Zhou, X.Y., andGuo, H.S.*(2011). Satellite RNA reduces expression of the 2b suppressor protein resulting in the attenuation of symptoms caused by Cucumber mosaic virus infection.Molecular plant pathology12, 595-605.
39.    Zhang, Z., Chen, H., Huang, X., Xia, R., Zhao, Q., Lai, J., Teng, K., Li, Y., Liang, L., Du, Q., Zhou, X.,Guo, H., and Xie, Q.* (2011). BSCTV C2 attenuates the degradation of SAMDC1 to suppress DNA methylation-mediated gene silencing in Arabidopsis.The Plant cell23, 273-288.
40.    Zhu, H., Duan, C.G., Hou, W.N., Du, Q.S., Lv, D.Q., Fang, R.X., andGuo, H.S.*(2011). Satellite RNA-derived small interfering RNA satsiR-12 targeting the 3' untranslated region of Cucumber mosaic virus triggers viral RNAs for degradation.Journal of virology85, 13384-13397.
41.    Chen, H., Zhang, Z., Teng, K., Lai, J., Zhang, Y., Huang, Y., Li, Y., Liang, L., Wang, Y., Chu, C.,Guo, H., and Xie, Q.* (2010). Up-regulation of LSB1/GDU3 affects geminivirus infection by activating the salicylic acid pathway.The Plant journal62, 12-23.
42.    Gao, F.#, Zhou, B.J.#, Li, G.Y., Jia, P.S., Li, H., Zhao, Y.L., Zhao, P., Xia, G.X., andGuo, H.S.*(2010). A glutamic acid-rich protein identified in Verticillium dahliae from an insertional mutagenesis affects microsclerotial formation and pathogenicity.PloS one5, e15319.
43.    Li, Y., Liu, X., Huang, L.,Guo, H.S.*, and Wang, X.J.* (2010). Potential coexistence of both bacterial and eukaryotic small RNA biogenesis and functional related protein homologs in Archaea.Journal of Genetics and Genomics37, 493-503.
44.    Liu, L.J., Zhang, Y.Y., Tang, S.Y., Zhao, Q.Z., Zhang, Z.H., Zhang, H.W., Dong, L.,Guo, H.S., and Xie, Q.* (2010). An efficient system to detect protein ubiquitination by agroinfiltration in Nicotiana benthamiana.The Plant journal61, 893-903.
45.    Teng, K.L., Chen, H., Lai, J.B., Zhang, Z.H., Fang, Y.Y., Xia, R., Zhou, X.P.,Guo, H.S., and Xie, Q.* (2010). Involvement of C4 protein of beet severe curly top virus (family Geminiviridae) in virus movement.PloS one5, e11280.
46.    Wang, W.Q., Wu, Y.R., Li, Y., Xie, J.Y., Zhang, Z.H., Deng, Z.Y., Zhang, Y.Y., Yang, C.P., Lai, J.B., Zhang, H.W., Bao, H.Y., Tang, S.Y., Yang, C.W., Gao, P., Xia, G.X.,Guo, H.S., and Xie, Q. *(2010). A large insert Thellungiella halophila BIBAC library for genomics and identification of stress tolerance genes.Plant molecular biology72, 91-99.
47.    Ying, X.B., Dong, L., Zhu, H., Duan, C.G., Du, Q.S., Lv, D.Q., Fang, Y.Y., Garcia, J.A., Fang, R.X., andGuo, H.S.*(2010). RNA-dependent RNA polymerase 1 from Nicotiana tabacum suppresses RNA silencing and enhances viral infection in Nicotiana benthamiana.The Plant cell22, 1358-1372.
48.    Lai, J.B., Chen, H., Teng, K.L., Zhao, Q.Z., Zhang, Z.H., Li, Y., Liang, L., Xia, R., Wu, Y.R.,Guo, H.S., and Xie, Q.* (2009). RKP, a RING finger E3 ligase induced by BSCTV C4 protein, affects geminivirus infection by regulation of the plant cell cycle.The Plant journal57, 905-917.
49.    Zheng, N.Y., Xia, R., Yang, C.W., Yin, B., Li, Y., Duan, C.G., Liang, L.,Guo, H.S., and Xie, Q.*(2009). Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice.Vaccine27, 5001-5007.
50.    Duan, C.G., Wang, C.H., Fang, R.X., andGuo, H.S.*(2008). Artificial MicroRNAs highly accessible to targets confer efficient virus resistance in plants.Journal of virology82, 11084-11095.
51.    He, X.F., Fang, Y.Y., Feng, L., andGuo, H.S.*(2008). Characterization of conserved and novel microRNAs and their targets, including a TuMV-induced TIR–NBS–LRR class R gene-derived novel miRNA in Brassica.FEBS letters582, 2445-2452.
52.    Zhang, Y.Y., Li, Y., Gao, T., Zhu, H., Wang, D.J., Zhang, H.W., Ning, Y.S., Liu, L.J., Wu, Y.R., Chu, C.C.,Guo, H.S., and Xie, Q.* (2008). Arabidopsis SDIR1 enhances drought tolerance in crop plants.Bioscience, biotechnology, and biochemistry72, 2251-2254.
53.    Du, Q.S.#, Duan, C.G.#, Zhang, Z.H.#, Fang, Y.Y., Fang, R.X., Xie, Q., andGuo, H.S.*(2007). DCL4 targets Cucumber mosaic virus satellite RNA at novel secondary structures.Journal of virology81, 9142-9151.
54.    Zhang, Y.Y., Yang, C.W., Li, Y., Zheng, N.Y., Chen, H., Zhao, Q.Z., Gao, T.,Guo, H.S., and Xie, Q.* (2007). SDIR1 is a RING finger E3 ligase that positively regulates stress-responsive abscisic acid signaling in Arabidopsis.The Plant cell19, 1912-1929.
55.    Zhang, Z.H., Xie, Q., andGuo, H.S.*(2007). Antiviral defense in plants.Plant Viruses1, 21-26.
56.    Xie, Q., andGuo, H.S.*(2006). Systemic antiviral silencing in plants.Virus research118, 1-6.
57.    Guo, H.S., Xie, Q., Fei, J.F., and Chua, N.H*. (2005). MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for arabidopsis lateral root development.The Plant cell17, 1376-1386.}