Principal Investigator：Prof. Wenbin Du   Telephone/Fax：+86 (0) 10-8299 4195 E-mail：wenbin(at)im.ac.cnAdd.:Institute of Microbiology Chinese Academy of Science No.7 Beitucheng West Road, Room G-303 Chaoyang District, Beijing 100029 China

Research Field   Microfluidics and Analytical Microbiology Applications

Research Interests Our research spans a broad range of Microbiology, Biochemistry, and Microfluidics. We use microfluidic droplets for high-throughput culturing and screening of single cells in nanoliter to picoliter volumes. We are developing automated workflows and devices for large scale single-cell isolation, cultivation, interaction and sequencing. We want to provide better approaches to improve the cultivation of microbial samples, to isolate and study rare microbes and extremophiles from the environments, to study the diversity, functions and evolution of complex microbial communities, and to advance clinical pathogen diagnosis and biosafety testing. Our current ongoing projects include: 1. Single-cell isolation, cultivation and sorting using high-throughput droplet microfluidic technologies. 2. Single cell whole genome amplification of uncultured microorganisms. 3. Digial Nucleic Acid Amplification and applications. 4. Isolation, identification and antimicrobial susceptibility testing of pathogens in clinical and environmental samples.

Lab Members       Principal Investigator Wenbin Du, Ph.D. Professor Email: wenbin(at)im.ac.cn  Education 06/2002 B. Sc. in Chemistry (Zhejiang University, China) 09/2002-06/2007Ph. D. in Chemistry (Zhejiang University, China) with Prof. Zhaolun Fang and Prof. Qun Fang Professional Experience 09/2007-08/2011 Postdoctoral Fellow (The University of Chicago) with Prof. Rustem F. Ismagilov 09/2011-03/2013 Principle Investigator, Renmin University of China, Department of Chemistry 03/2013-Present  Professor, State Key Laboratory of Microbial Resources, The Institute of Microbiology, Chinese Academy of Sciences 6/2016- Present   Professor, Savaid Medical School, University of the Chinese Academy of Sciences,   Permanent staff  Lan, Ying  PH.D. Senior Engineer, Institute of Microbiology, CAS, 2008-  Associate Professor, Institute of Genetics and Developmental Biology, CAS, 2004-2008  Associate Professor, Jilin Medical College Beihua University, 1991-2004  PhD in Cell Biology, Northeast Normal University, 2001-2004  Email: lanying@im.ac.cn Chen, Dongwei  PH.D.  Assistant Professor, Institute of Microbiology, CAS, 2015-  PhD in Microbiology, Institute of Microbiology, CAS, 2007-2013  Email: chendw@im.ac.cn Qiao, Yuxin  PH.D.  Assistant Professor, Institute of Microbiology, CAS, 2019-  PhD in Microbiology, Institute of Microbiology, CAS, 2014.9-2019.6  BSc in Biology, Central South University, 2010-2014 Email: qiaoyx@im.ac.cn Wang, Jian  PH.D.  Assistant Professor, Institute of Microbiology, CAS, 2013-  Post-doc Fellow, Institute of Microbiology, CAS, Microbiology, 2009-2013  PhD in Microbiology,South China Sea Institute of Oceanology, CAS, 2006-2009  Email: wangj@im.ac.cn Yun, Juanli  PH.D. Assistant Professor, Institute of Microbiology, CAS, 2015-  Post-doc Fellow, University of Chinese Academy of Sciences, 2012-2015  PhD in Environmental Science, University of Chinese Academy of Sciences, 2008-2011  Email: yunjl@im.ac.cn Zhou, Ziwei  PH.D. Research assistant, Institute of Microbiology, CAS, 2019.8-  BSc in Biotechnology, Beijing City University, 2015-2019  Email: zzw_dtz@126.com   Graduate Students  Hu, Beiyu PhD Candidate, 2016-  BSc, Henan Agricultural University, 2012-2016  Email:huby@im.ac.cn Hu, Ran Master, 2017-  BSc, Dalian Minzu University, 2013-2017  Email:raner0409@qq.com Li, Caiming Master, 2018-  BSc, BeiJing Forestry University, 2014-2018  Email: xiaonansu@outlook.com Tao, Yi PhD Candidate, 2015-  BSc, Northwest A&F University, 2011-2015  Email: taoyi19911010@126.com Xie, Bingliang PhD Candidate, 2018-  BSc, BeiJing Forestry University, 2014-2018  Email:xiebl@im.ac.cn Yu, Haiyan  Master, 2019-  Beijing University of Agriculture, Undergraduate, 2015-2019.6  Email: yu--haiyan@outlook.com Zheng, Meng  Master, 2017-  BSc, Shandong Normal University, 2013-2017  Email: zhengmengsunny@yeah.net   Undergraduate Students Yu, Yanghuan  ZheJiang University，Undergraduate, 2016-  Email:3160104948@zju.edu.cn Wang, Zhiyi BeiJing Forestry University, Undergraduate, 2016-  Email: jaywang19970429@163.com

Selective Publications   1. Yi, Q.L.#; Cai, D.Y.#; Xiao, M.; Nie, M.Y.; Cui, Q.N.; Cheng, J.W.; Li, C.M.; Feng, J.; Urban, G.; Xu, Y.-C.*; Lan, Y.*; Du, W.B.* Direct Antimicrobial Susceptibility Testing of Bloodstream Infection on SlipChip, Biosensors & Bioelectronics, 2019, 135, 200-207. 2. Nie, M.Y.; Zheng, M.; Li, C.M.; Shen, F.; Liu, M.H.; Luo, H.B.; Song, X.H.; Lan, Y.*; Pan, J.Z.*; Du, W.B.* Assembled Step Emulsification Device for Multiplex Droplet Digital PCR, Analytical Chemistry, 2019, 91, 1779–1784. 3. Chen, D.W.; Liu, S.-J.; Du, W.B.* Chemotactic screening of imidazolinone-degrading bacteria by microfluidic SlipChip, Journal of Hazardous Materials, 2019, 366, 512-519. 4. Jin, Z.J.; Nie, M.Y.; Zhao, T.H.; Xu, J.Y.; Chen, D.W.; Yun, J.L.; Hu, R.; Ma, L.Z.; Du, W.B.* Dynamic Sessile-droplet Habitats for Controllable Cultivation of Bacterial Biofilm, Small, 2018, 14, 1800658.PDF 5. Qiao, Y.X.; Zhao, X.Y.; Zhu, J.; Tu, R.; Dong, L.B.; Wang, L.; Dong, Z.Y.; Wang, Q.H.; Du, W.B.* Fluorescence-Activated Droplet Sorting of Lipolytic Microorganisms Using a Compact Optical System, Lab On a Chip, 2018, 18, 190-196. 6. Liao, S.L; Tao, X.L.; Ju, Y.J.; Feng, J.*; Du, W.B.*; Wang, Y.P.* Multi-Channel Dynamic Interfacial Printing: An Alternative Multi-Component Droplet Generation Technique for Lab in a Drop, ACS Applied Materials and Interfaces, 2017, 9, 4345-4352. 7. Hu, Y.#; Xu, P.#; Luo, J.; He, H.X.*; Du, W.B.* Absolute Quantification of H5-subtype Avian Influenza Viruses Using Droplet Digital Loop-mediated Isothermal Amplification, Analytical Chemistry, 2017, 89 (1), 745-750. (#Equal contribution). 8. Xu, P.; Zheng, X.; Tao, Y.; Du, W.B.* Cross-Interface Emulsification for Generating Size-Tuneable Droplets, Analytical Chemistry, 2016, 88, 3171-3177. 9. Jiang, C.Y.; Dong, L.B.; Zhao, J.K.; Hu, X.F.; Shen, C.H.; Qiao, Y.X.; Zhang, X.Y.; Wang, Y.P.; Ismagilov, R.F.; Liu, S.J.*; Du, W.B.* High throughput Single-cell Cultivation on Microfluidic Streak Plates, Applied Environmental Microbiology, 2016, 82, 2210-2218. 10. Liu, X.; Yi, Q.L.; Han, Y.Z.; Liang, Z.N.; Shen, C.H.; Zhou, Z.Y.; Sun J.L.; Li, Y.Z.; Du, W.B.*; Cao, R.* A Robust Microfluidic Device for Synthesis and Crystal Growth of Organometallic Polymers with Highly Organized Structures, Angewandte Chemie International Edition, 2015, 54, 1846-1850. 11. Cai, D.Y.; Xiao, M.; Xu, P.; Xu, Y.C.*; Du, W.B.* An Integrated Microfluidic Device Utilizing Dielectrophoresis and Multiplex Array PCR for Point-of-Care Detection of Pathogens, Lab On a Chip, 2014, 14, 3917-3924. (HOT Article, Front Cover) 12. Shen, C.H.; Xu, P.; Huang, Z.; Liu, S.J.*; Du, W.B.* Bacterial Chemotaxis on SlipChip, Lab On a Chip, 2014, 14, 3074-3080. 13. Wang, S.J.; Chen, S.M.; Wang, J.N.; Xu, P.; Luo, Y.M.; Nie, Z.X.*; Du, W. B.* Interface Solution Isoelectric Focusing with in situ MALDI-TOF Mass Spectrometry, Electrophoresis, 2014, 35, 2528-2533. 14. Du, W.B.#; Sun, M.#; Gu, S.Q.#; Zhu, Y.; Fang, Q.* Automated Microfluidic Screening Assay Platform Based on DropLab, Analytical Chemistry, 2010, 82, 9941-9947 (#Equal contribution). 15. Li, L.; Du, W.B.*; Ismagilov, R.F.* Multiparameter Screening on SlipChip Used for Nanoliter Protein Crystallization Combining Free Interface Diffusion and Microbatch Methods, Journal of the American Chemical Society, 2010, 132, 112-119. 16. Shen, F.; Du, W.B.; Kreutz, J.E.; Fok, A.; Ismagilov, R.F.* Digital PCR on a SlipChip, Lab on a Chip, 2010, 10, 2666-2672. 17. Du, W.B.; Li, L.; Nichols, K.P.; Ismagilov, R.F.* SlipChip, Lab on a Chip, 2009, 9, 2286-2292. 18. Chen, D.L.#; Du, W.B.#; Liu, Y.#; Liu, W.S.#; Kuznetsov, A.; Mendez, F.E.; Philipson, L.H.; Ismagilov, R.F.* The chemistrode: A Droplet-based Microfluidic Device for Stimulation and Recording with High Temporal, Spatial, and Chemical Resolution, Proceedings of the National Academy of Sciences of the United States of America, 2008, 105, 16843-16848 (#Equal contribution). 19. Du, W.B.; Fang, Q.*; Fang, Z.L. Microfluidic Sequential Injection Analysis in a Short Capillary, Analytical Chemistry, 2006, 78, 6404-6410. 20. Du, W.B.; Fang, Q.*; He, Q.H.; Fang, Z.L. High-throughput Nanoliter Sample Introduction Microfluidic Chip-based Flow Injection Analysis System with Gravity-driven Flows, Analytical Chemistry, 2005, 77, 1330-1337.