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Analytical Microbiology Research Group




Principal Investigator:Prof. Wenbin Du


Telephone/Fax:+86 (0) 10-8299 4195
E-mail:wenbin(at)im.ac.cnAdd.:Institute of Microbiology Chinese Academy of Science No.7 Beitucheng West Road, Room G-303 Chaoyang District, Beijing 100029 China 


Research Field  

Microfluidics and Analytical Microbiology Applications

Research Interests

Our research spans a broad range of Microbiology, Biochemistry, and Microfluidics. We use microfluidic droplets for high-throughput culturing and screening of single cells in nanoliter to picoliter volumes. We are developing automated workflows and devices for large scale single-cell isolation, cultivation, interaction and sequencing. We want to provide better approaches to improve the cultivation of microbial samples, to isolate and study rare microbes and extremophiles from the environments, to study the diversity, functions and evolution of complex microbial communities, and to advance clinical pathogen diagnosis and biosafety testing. Our current ongoing projects include:

1. Single-cell isolation, cultivation and sorting using high-throughput droplet microfluidic technologies.

2. Single cell whole genome amplification of uncultured microorganisms.

3. Digial Nucleic Acid Amplification and applications.

4. Isolation, identification and antimicrobial susceptibility testing of pathogens in clinical and environmental samples.


Lab Members  



Principal Investigator

Wenbin Du, Ph.D.


Email: wenbin(at)im.ac.cn 

06/2002 B. Sc. in Chemistry (Zhejiang University, China)
09/2002-06/2007Ph. D. in Chemistry (Zhejiang University, China) with Prof. Zhaolun Fang and Prof. Qun Fang

Professional Experience
09/2007-08/2011 Postdoctoral Fellow (The University of Chicago) with Prof. Rustem F. Ismagilov
09/2011-03/2013 Principle Investigator, Renmin University of China, Department of Chemistry
03/2013-Present  Professor, State Key Laboratory of Microbial Resources, The Institute of Microbiology, Chinese Academy of Sciences
6/2016- Present   Professor, Savaid Medical School, University of the Chinese Academy of Sciences,


Permanent staff 

Lan, Ying  PH.D.

Senior Engineer, Institute of Microbiology, CAS, 2008- 
Associate Professor, Institute of Genetics and Developmental Biology, CAS, 2004-2008 
Associate Professor, Jilin Medical College Beihua University, 1991-2004 
PhD in Cell Biology, Northeast Normal University, 2001-2004 
Email: lanying@im.ac.cn

Chen, Dongwei  PH.D. 

Assistant Professor, Institute of Microbiology, CAS, 2015- 
PhD in Microbiology, Institute of Microbiology, CAS, 2007-2013 
Email: chendw@im.ac.cn

Qiao, Yuxin  PH.D. 

Assistant Professor, Institute of Microbiology, CAS, 2019- 
PhD in Microbiology, Institute of Microbiology, CAS, 2014.9-2019.6 
BSc in Biology, Central South University, 2010-2014
Email: qiaoyx@im.ac.cn

Wang, Jian  PH.D. 

Assistant Professor, Institute of Microbiology, CAS, 2013- 
Post-doc Fellow, Institute of Microbiology, CAS, Microbiology, 2009-2013 
PhD in Microbiology,South China Sea Institute of Oceanology, CAS, 2006-2009 
Email: wangj@im.ac.cn

Yun, Juanli  PH.D.

Assistant Professor, Institute of Microbiology, CAS, 2015- 
Post-doc Fellow, University of Chinese Academy of Sciences, 2012-2015 
PhD in Environmental Science, University of Chinese Academy of Sciences, 2008-2011 
Email: yunjl@im.ac.cn

Zhou, Ziwei  PH.D. Research assistant, Institute of Microbiology, CAS, 2019.8- 
BSc in Biotechnology, Beijing City University, 2015-2019 
Email: zzw_dtz@126.com


Graduate Students 

Hu, Beiyu

PhD Candidate, 2016- 
BSc, Henan Agricultural University, 2012-2016 

Hu, Ran

Master, 2017- 
BSc, Dalian Minzu University, 2013-2017 

Li, Caiming

Master, 2018- 
BSc, BeiJing Forestry University, 2014-2018 
Email: xiaonansu@outlook.com

Tao, Yi

PhD Candidate, 2015- 
BSc, Northwest A&F University, 2011-2015 
Email: taoyi19911010@126.com

Xie, Bingliang

PhD Candidate, 2018- 
BSc, BeiJing Forestry University, 2014-2018 

Yu, Haiyan 

Master, 2019- 
Beijing University of Agriculture, Undergraduate, 2015-2019.6 
Email: yu--haiyan@outlook.com

Zheng, Meng 

Master, 2017- 
BSc, Shandong Normal University, 2013-2017 
Email: zhengmengsunny@yeah.net


Undergraduate Students

Yu, Yanghuan 

ZheJiang University,Undergraduate, 2016- 

Wang, Zhiyi

BeiJing Forestry University, Undergraduate, 2016- 
Email: jaywang19970429@163.com



Selective Publications


1. Yi, Q.L.#; Cai, D.Y.#; Xiao, M.; Nie, M.Y.; Cui, Q.N.; Cheng, J.W.; Li, C.M.; Feng, J.; Urban, G.; Xu, Y.-C.*; Lan, Y.*; Du, W.B.* Direct Antimicrobial Susceptibility Testing of Bloodstream Infection on SlipChip, Biosensors & Bioelectronics, 2019, 135, 200-207.

2. Nie, M.Y.; Zheng, M.; Li, C.M.; Shen, F.; Liu, M.H.; Luo, H.B.; Song, X.H.; Lan, Y.*; Pan, J.Z.*; Du, W.B.* Assembled Step Emulsification Device for Multiplex Droplet Digital PCR, Analytical Chemistry, 2019, 91, 1779–1784.

3. Chen, D.W.; Liu, S.-J.; Du, W.B.* Chemotactic screening of imidazolinone-degrading bacteria by microfluidic SlipChip, Journal of Hazardous Materials, 2019, 366, 512-519.

4. Jin, Z.J.; Nie, M.Y.; Zhao, T.H.; Xu, J.Y.; Chen, D.W.; Yun, J.L.; Hu, R.; Ma, L.Z.; Du, W.B.* Dynamic Sessile-droplet Habitats for Controllable Cultivation of Bacterial Biofilm, Small, 2018, 14, 1800658.PDF

5. Qiao, Y.X.; Zhao, X.Y.; Zhu, J.; Tu, R.; Dong, L.B.; Wang, L.; Dong, Z.Y.; Wang, Q.H.; Du, W.B.* Fluorescence-Activated Droplet Sorting of Lipolytic Microorganisms Using a Compact Optical System, Lab On a Chip, 2018, 18, 190-196.

6. Liao, S.L; Tao, X.L.; Ju, Y.J.; Feng, J.*; Du, W.B.*; Wang, Y.P.* Multi-Channel Dynamic Interfacial Printing: An Alternative Multi-Component Droplet Generation Technique for Lab in a Drop, ACS Applied Materials and Interfaces, 2017, 9, 4345-4352.

7. Hu, Y.#; Xu, P.#; Luo, J.; He, H.X.*; Du, W.B.* Absolute Quantification of H5-subtype Avian Influenza Viruses Using Droplet Digital Loop-mediated Isothermal Amplification, Analytical Chemistry, 2017, 89 (1), 745-750. (#Equal contribution).

8. Xu, P.; Zheng, X.; Tao, Y.; Du, W.B.* Cross-Interface Emulsification for Generating Size-Tuneable Droplets, Analytical Chemistry, 2016, 88, 3171-3177.

9. Jiang, C.Y.; Dong, L.B.; Zhao, J.K.; Hu, X.F.; Shen, C.H.; Qiao, Y.X.; Zhang, X.Y.; Wang, Y.P.; Ismagilov, R.F.; Liu, S.J.*; Du, W.B.* High throughput Single-cell Cultivation on Microfluidic Streak Plates, Applied Environmental Microbiology, 2016, 82, 2210-2218.

10. Liu, X.; Yi, Q.L.; Han, Y.Z.; Liang, Z.N.; Shen, C.H.; Zhou, Z.Y.; Sun J.L.; Li, Y.Z.; Du, W.B.*; Cao, R.* A Robust Microfluidic Device for Synthesis and Crystal Growth of Organometallic Polymers with Highly Organized Structures, Angewandte Chemie International Edition, 2015, 54, 1846-1850.

11. Cai, D.Y.; Xiao, M.; Xu, P.; Xu, Y.C.*; Du, W.B.* An Integrated Microfluidic Device Utilizing Dielectrophoresis and Multiplex Array PCR for Point-of-Care Detection of Pathogens, Lab On a Chip, 2014, 14, 3917-3924. (HOT Article, Front Cover)

12. Shen, C.H.; Xu, P.; Huang, Z.; Liu, S.J.*; Du, W.B.* Bacterial Chemotaxis on SlipChip, Lab On a Chip, 2014, 14, 3074-3080.

13. Wang, S.J.; Chen, S.M.; Wang, J.N.; Xu, P.; Luo, Y.M.; Nie, Z.X.*; Du, W. B.* Interface Solution Isoelectric Focusing with in situ MALDI-TOF Mass Spectrometry, Electrophoresis, 2014, 35, 2528-2533.

14. Du, W.B.#; Sun, M.#; Gu, S.Q.#; Zhu, Y.; Fang, Q.* Automated Microfluidic Screening Assay Platform Based on DropLab, Analytical Chemistry, 2010, 82, 9941-9947 (#Equal contribution).

15. Li, L.; Du, W.B.*; Ismagilov, R.F.* Multiparameter Screening on SlipChip Used for Nanoliter Protein Crystallization Combining Free Interface Diffusion and Microbatch Methods, Journal of the American Chemical Society, 2010, 132, 112-119.

16. Shen, F.; Du, W.B.; Kreutz, J.E.; Fok, A.; Ismagilov, R.F.* Digital PCR on a SlipChip, Lab on a Chip, 2010, 10, 2666-2672.

17. Du, W.B.; Li, L.; Nichols, K.P.; Ismagilov, R.F.* SlipChip, Lab on a Chip, 2009, 9, 2286-2292.

18. Chen, D.L.#; Du, W.B.#; Liu, Y.#; Liu, W.S.#; Kuznetsov, A.; Mendez, F.E.; Philipson, L.H.; Ismagilov, R.F.* The chemistrode: A Droplet-based Microfluidic Device for Stimulation and Recording with High Temporal, Spatial, and Chemical Resolution, Proceedings of the National Academy of Sciences of the United States of America, 2008, 105, 16843-16848 (#Equal contribution).

19. Du, W.B.; Fang, Q.*; Fang, Z.L. Microfluidic Sequential Injection Analysis in a Short Capillary, Analytical Chemistry, 2006, 78, 6404-6410.

20. Du, W.B.; Fang, Q.*; He, Q.H.; Fang, Z.L. High-throughput Nanoliter Sample Introduction Microfluidic Chip-based Flow Injection Analysis System with Gravity-driven Flows, Analytical Chemistry, 2005, 77, 1330-1337.




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Institute Of Microbiology Chinese Academy of Sciences
NO.1 West Beichen Road, Chaoyang District, Beijing 100101, China Phone: 0086-10-64807462 Fax: 0086-10-64807468 Email: office@im.ac.cn