Principal Investigators
State Key Laboratory of Microbial Resources
State Key Laboratory of Mycology
CAS Key laboratory of Microbial Physiological and Metabolic Engineering
State Key Laboratory of Plant Genomics(Co-established with Institute of Genetics and Developmental Biology, CAS)
CAS Key laboratory of Pathogenic Microbiology & Immunology
The Laboratory of Microbiome and Microecological Technology
The Center for Microbial Resource and Big Data-
State Key Laboratory of Microbial Resources
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Principal Investigator,Professor : Microbial elements mining and systematic metabolic engineering

Wang Weishan
Research Field：Room H611, NO.1 West Beichen Road, Chaoyang District, Beijing 100101, China
Telephone/fax：+86-10-64807800
E-mail：wangws@im.ac.cn
Research Interests
Our lab dedicates to elucidating the diverse physiological and metabolic mechanisms of microorganisms and advancing enabling technologies for biomanufacturing. Specifically, we first conduct systematic investigations into the metabolic and regulatory principles of microorganisms to uncover their metabolic diversity. Subsequently, leveraging this knowledge, we devise innovative strategies for metabolic engineering, directed evolution, and efficient screening of high-yield strains, thereby developing industrial strains in a demand-driven manner. Ultimately, our work bridges the gap between fundamental research and industrial application. 
Education/degrees
2013 Ph.D., Biochemistry and Molecular Biology, University of Chinese Academy of Sciences, Beijing, China
2009 Master of Medicine, Ocean University of China, Qingdao, China
2006 Bachelor of Science, Shandong Agricultural University, Tai’an, China
Work experience
2019-Present
Principle Investigator, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
2016-2019
Associate Professor, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
2013-2016
Assistant professor, Institute for Microbiology, Chinese Academy of Sciences, Beijing, China
Publication

2025

Yan, H.; Xin, Z.; Sang, Z.; Li, X.; Xie, J.; Wu, J.; Pang, S.; Wen, Y.; Wang, W. A Rational Multi-Target Combination Strategy for Synergistic Improvement of Non-Ribosomal Peptide Production. Nat. Commun. 2025, 16 (1). https://doi.org/10.1038/s41467-025-57073-5.

Wang, H.; Wang, L.; Li, D.; Fan, K.; Yang, Y.; Cao, H.; Sun, J.; Ren, J.; Liu, Y.; Xiang, L.; Li, W.; Pan, M.; Hu, H.; Chen, Y.; Xu, Z.; Huang, Y.; Wang, W.; Pan, G. Uncovering the Molecular Landscape of Tetracycline Family Natural Products through Bacterial Genome Mining. J. Am. Chem. Soc. 2025, 147 (18), 15100–15114. https://doi.org/10.1021/jacs.4c17551.

Li, B.; Yang, Z.; Li, Z.; Zhang, Y.; Zhang, L.; Wang, W. Enabling Genetic Manipulation and Robustness of Bacillus Methanolicus for Methanol-Based Bio-Manufacturing. Metab. Eng. 2025, 89, 121–134. https://doi.org/10.1016/j.ymben.2025.02.013.

Yan, H.; Li, S.; Wang, W. Reprogramming Naturally Evolved Switches for Streptomyces Chassis Development. Trends Biotechnol. 2025, 43 (1), 12–15. https://doi.org/10.1016/j.tibtech.2024.07.001.

2024

He, X.; Wang, D.; Liu, J.; Shi, T.; Wang, W.; Chen, B.; Li, D.; Zhang, L.; Tan, G. Y. Engineering the Methylerythritol Phosphate Pathway and Using a Temporal Promoter for Enhanced Lycopene Production in Rhodobacter Sphaeroides HY01. J. Agric. Food Chem. 2024. https://doi.org/10.1021/ACS.JAFC.4C07848/SUPPL_FILE/JF4C07848_SI_001.PDF.

Yang, Z.; Li, B.; Bu, R.; Wang, Z.; Xin, Z.; Li, Z.; Zhang, L.; Wang, W. A Highly Efficient Method for Genomic Deletion across Diverse Lengths in Thermophilic Parageobacillus Thermoglucosidasius. Synth. Syst. Biotechnol. 2024, 9 (4), 658–666. https://doi.org/10.1016/j.synbio.2024.05.009.

Zhou, H.; Zhang, C.; Li, Z.; Xia, M.; Li, Z.; Wang, Z.; Tan, G. Y.; Luo, Y.; Zhang, L.; Wang, W. Systematic Development of a Highly Efficient Cell Factory for 5-Aminolevulinic Acid Production. Trends Biotechnol. 2024. https://doi.org/10.1016/j.tibtech.2024.06.004.

Zhang, M.; Shi, H.; Wang, X.; Zhu, Y.; Li, Z.; Tu, L.; Zheng, Y.; Xia, M.; Wang, W.; Wang, M. AI-Based Automated Construction of High-Precision Geobacillus Thermoglucosidasius Enzyme Constraint Model. Metab. Eng. 2024, 86, 208–233. https://doi.org/10.1016/j.ymben.2024.10.006.

Wang, J.; Wang, W.; Chen, Y.; Liu, Z.; Ji, X.; Pan, G.; Li, Z.; Fan, K. Development of a Xylose-Inducible and Glucose-Insensitive Expression System for Parageobacillus Thermoglucosidasius. Appl. Microbiol. Biotechnol. 2024, 108 (1), 493. https://doi.org/10.1007/s00253-024-13333-w.

Wang, S.; Zeng, X.; Jiang, Y.; Wang, W.; Bai, L.; Lu, Y.; Zhang, L.; Tan, G. Y. Unleashing the Potential: Type I CRISPR-Cas Systems in Actinomycetes for Genome Editing. Nat. Prod. Rep. 2024. https://doi.org/10.1039/d4np00010b.

Sang, Z.; Li, X.; Yan, H.; Wang, W.; Wen, Y. Development of a Group II Intron-Based Genetic Manipulation Tool for Streptomyces. Microb. Biotechnol. 2024, 17 (5). https://doi.org/10.1111/1751-7915.14472.

Qiu, S.; Yang, B.; Li, Z.; Li, S.; Yan, H.; Xin, Z.; Liu, J.; Zhao, X.; Zhang, L.; Xiang, W.; Wang, W. Building a Highly Efficient Streptomyces Super-Chassis for Secondary Metabolite Production by Reprogramming Naturally-Evolved Multifaceted Shifts. Metab. Eng. 2024, 81, 210–226. https://doi.org/10.1016/j.ymben.2023.12.007.

Shi, X.; Yan, H.; Yuan, F.; Li, G.; Liu, J.; Li, C.; Yu, X.; Li, Z.; Zhu, Y.; Wang, W. LexA, an SOS Response Repressor, Activates TGase Synthesis in Streptomyces Mobaraensis. Front. Microbiol. 2024, 15. https://doi.org/10.3389/fmicb.2024.1397314.

Chen, H.; Wang, Y.; Wang, W.; Cao, T.; Zhang, L.; Wang, Z.; Chi, X.; Shi, T.; Wang, H.; He, X.; Liang, M.; Yang, M.; Jiang, W.; Lv, D.; Yu, J.; Zhu, G.; Xie, Y.; Gao, B.; Wang, X.; Liu, X.; Li, Y.; Ouyang, L.; Zhang, J.; Liu, H.; Li, Z.; Tong, Y.; Xia, X.; Tan, G. Y.; Zhang, L. High-Yield Porphyrin Production through Metabolic Engineering and Biocatalysis. Nat. Biotechnol. 2024. https://doi.org/10.1038/s41587-024-02267-3.

2023

Yang, Z.; Li, Z.; Li, B.; Bu, R.; Tan, G. Y.; Wang, Z.; Yan, H.; Xin, Z.; Zhang, G.; Li, M.; Xiang, H.; Zhang, L.; Wang, W. A Thermostable Type I-B CRISPR-Cas System for Orthogonal and Multiplexed Genetic Engineering. Nat. Commun. 2023, 14 (1). https://doi.org/10.1038/s41467-023-41973-5.

Li, S.; Li, Z.; Tan, G. Y.; Xin, Z.; Wang, W. In Vitro Allosteric Transcription Factor-Based Biosensing. Trends Biotechnol. 2023, 41 (8), 1080–1095. https://doi.org/10.1016/j.tibtech.2023.03.001.

2022

Wang, J.; Li, Z.; Wang, W.; Pang, S.; Yao, Y.; Yuan, F.; Wang, H.; Xu, Z.; Pan, G.; Liu, Z.; Chen, Y.; Fan, K. Dynamic Control Strategy to Produce Riboflavin with Lignocellulose Hydrolysate in the Thermophile Geobacillus Thermoglucosidasius. ACS Synth. Biol. 2022, 11 (6), 2163–2174. https://doi.org/10.1021/acssynbio.2c00087.

Liang, M.; Liu, L.; Xu, F.; Zeng, X.; Wang, R.; Yang, J.; Wang, W.; Karthik, L.; Liu, J.; Yang, Z.; Zhu, G.; Wang, S.; Bai, L.; Tong, Y.; Liu, X.; Wu, M.; Zhang, L.-X.; Tan, G.-Y. Activating Cryptic Biosynthetic Gene Cluster through a CRISPR–Cas12a-Mediated Direct Cloning Approach. Nucleic Acids Res. 2022, 50 (6), 3581–3592. https://doi.org/10.1093/nar/gkac181.

Li, Z.; Zhang, C.; Wang, Z.; Li, C.; Yang, Z.; Li, Z.; Zhang, L.; Wang, W. High-Throughput and Reliable Acquisition of in Vivo Turnover Number Fuels Precise Metabolic Engineering. Synth. Syst. Biotechnol. 2022, 7 (1), 541–543. https://doi.org/10.1016/j.synbio.2021.12.006.

2021

Zhao, X.; Wang, Z.; Yang, B.; Li, Z.; Tong, Y.; Bi, Y.; Li, Z.; Xia, X.; Chen, X.; Zhang, L.; Wang, W.; Tan, G. Y. Integrating PCR-Free Amplification and Synergistic Sensing for Ultrasensitive and Rapid CRISPR/Cas12a-Based SARS-CoV-2 Antigen Detection. Synth. Syst. Biotechnol. 2021, 6 (4), 283–291. https://doi.org/10.1016/j.synbio.2021.09.007.

Li, S.; Yang, B.; Tan, G. Y.; Ouyang, L. M.; Qiu, S.; Wang, W.; Xiang, W.; Zhang, L. Polyketide Pesticides from Actinomycetes. Curr. Opin. Biotechnol. 2021, 69, 299–307. https://doi.org/10.1016/J.COPBIO.2021.05.006.

Li, S.; Li, Z.; Pang, S.; Xiang, W.; Wang, W. Coordinating Precursor Supply for Pharmaceutical Polyketide Production in Streptomyces. Curr. Opin. Biotechnol. 2021, 69, 26–34. https://doi.org/10.1016/j.copbio.2020.11.006.

Yang, Y.; Sun, Q.; Liu, Y.; Yin, H.; Yang, W.; Wang, Y.; Liu, Y.; Li, Y.; Pang, S.; Liu, W.; Zhang, Q.; Yuan, F.; Qiu, S.; Li, J.; Wang, X.; Fan, K.; Wang, W.; Li, Z.; Yin, S. Development of a pyrF-Based Counterselectable System for Targeted Gene Deletion in Streptomyces Rimosus. J. Zhejiang Univ. Sci. B 2021, 22 (5), 383–396. https://doi.org/10.1631/jzus.B2000606.

Yang, Z.; Sun, Q.; Tan, G.; Zhang, Q.; Wang, Z.; Li, C.; Qi, F.; Wang, W.; Zhang, L.; Li, Z. Engineering Thermophilic Geobacillus Thermoglucosidasius for Riboflavin Production. Microb. Biotechnol. 2021, 14 (2), 363–373. https://doi.org/10.1111/1751-7915.13543.

Shi, T.; Zhang, L.; Liang, M.; Wang, W.; Wang, K.; Jiang, Y.; Liu, J.; He, X.; Yang, Z.; Chen, H.; Li, C.; Lv, D.; Zhou, L.; Chen, B.; Li, D.; Zhang, L.-X.; Tan, G.-Y. Screening and Engineering of High-Activity Promoter Elements through Transcriptomics and Red Fluorescent Protein Visualization in Rhodobacter Sphaeroides. Synth. Syst. Biotechnol. 2021, 6, 335–342. https://doi.org/10.1016/j.synbio.2021.09.011.

2020

Cao, Z.; Yu, J.; Wang, W.; Lu, H.; Xia, X.; Xu, H.; Yang, X.; Bao, L.; Zhang, Q.; Wang, H.; Zhang, S.; Zhang, L. Multi-Scale Data-Driven Engineering for Biosynthetic Titer Improvement. Curr. Opin. Biotechnol. 2020, 65, 205–212. https://doi.org/10.1016/J.COPBIO.2020.04.002.

Zhang, Q.; Ren, J. W.; Wang, W.; Zhai, J.; Yang, J.; Liu, N.; Huang, Y.; Chen, Y.; Pan, G.; Fan, K. A Versatile Transcription-Translation in One Approach for Activation of Cryptic Biosynthetic Gene Clusters. ACS Chem. Biol. 2020, 15 (9), 2551–2557. https://doi.org/10.1021/acschembio.0c00581.

Wang, W.; Li, S.; Li, Z.; Zhang, J.; Fan, K.; Tan, G.; Ai, G.; Lam, S. M.; Shui, G.; Yang, Z.; Lu, H.; Jin, P.; Li, Y.; Chen, X.; Xia, X.; Liu, X.; Dannelly, H. K.; Yang, C.; Yang, Y.; Zhang, S.; Alterovitz, G.; Xiang, W.; Zhang, L. Harnessing the Intracellular Triacylglycerols for Titer Improvement of Polyketides in Streptomyces. Nat. Biotechnol. 2020, 38 (1), 76–83. https://doi.org/10.1038/s41587-019-0335-4.

2019
Wang X#, Yin S#, Bai J, Liu Y, Fan K, Wang H, Yuan F, Zhao B*, Li Z*, Wang W*: Heterologous production of chlortetracycline in an industrial grade Streptomyces rimosus host. Appl Microbiol Biotechnol 2019, 103(16):6645-6655.

Liang M#, Li Z#, Wang W#, Liu J#, Liu L, Zhu G, Karthik L, Wang M, Wang KF, Wang Z, Yu J, Shuai Y, Yu J, Zhang L, Yang Z, Li C, Zhang Q, Shi T, Zhou L, Xie F, Dai H, Liu X, Zhang J, Liu G, Zhuo Y, Zhang B, Liu C, Li S, Xia X, Tong Y, Liu Y, Alterovitz G, Tan G*, Zhang L*: A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules. Nat Commun 2019, 10(1):3672.

Wang J#, Wang W, Wang H, Yuan F, Xu Z, Yang K, Li Z, Chen Y*, Fan K*: Improvement of stress tolerance and riboflavin production of Bacillus subtilis by introduction of heat shock proteins from thermophilic bacillus strains. Appl Microbiol Biotechnol 2019, 103(11):4455-4465.

2018

Cao J#, Yao Y#, Fan K, Tan G, Xiang W, Xia X, Li S*, Wang W*, Zhang L*: Harnessing a previously unidentified capability of bacterial allosteric transcription factors for sensing diverse small molecules in vitro. Sci Adv 2018, 4(11):eaau4602.

Yao Y#, Li S#, Cao J, Liu W, Fan K, Xiang W, Yang K*, Kong D*, Wang W*: Development of small molecule biosensors by coupling the recognition of the bacterial allosteric transcription factor with isothermal strand displacement amplification. Chem Commun (Camb) 2018, 54(38):4774-4777.

Yao Y#, Li S#, Cao J, Liu W, Qi F, Xiang W, Yang K, Wang W*, Zhang L*: A novel signal transduction system for development of uric acid biosensors. Appl Microbiol Biotechnol 2018, 102(17):7489-7497.

Li S#, Wang J#, Xiang W, Yang K, Li Z*, Wang W*: An Autoregulated Fine-Tuning Strategy for Titer Improvement of Secondary Metabolites Using Native Promoters in Streptomyces. ACS Synth Biol 2018, 7(2):522-530.

2017

Li X#, Wang J, Shi M, Wang W, Corre C, Yang K*: Evidence for the formation of ScbR/ScbR2 heterodimers and identification of one of the regulatory targets in Streptomyces coelicolor. Appl Microbiol Biotechnol 2017, 101(13):5333-5340.

Li S#, Zhou L, Yao Y, Fan K, Li Z, Zhang L, Wang W*, Yang K*: A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays. Chem Commun (Camb) 2017, 53(1):99-102.

2016

Wang W#, Yang T#, Li Y, Li S, Yin S, Styles K, Corre C, Yang K*: Development of a Synthetic Oxytetracycline-Inducible Expression System for Streptomycetes Using de Novo Characterized Genetic Parts. ACS Synth Biol 2016, 5(7):765-773.

Yin S#, Li Z#, Wang X, Wang H, Jia X, Ai G, Bai Z, Shi M, Yuan F, Liu T, Wang W*, Yang K*: Heterologous expression of oxytetracycline biosynthetic gene cluster in Streptomyces venezuelae WVR2006 to improve production level and to alter fermentation process. Appl Microbiol Biotechnol 2016, 100(24):10563-10572.

Jiang T#, Ke B, Chen H, Wang W, Du L, Yang K, Li M*: Bioluminescent Probe for Detecting Mercury(II) in Living Mice. Anal Chem 2016, 88(15):7462-7465.

Sidda JD#, Poon V, Song L, Wang W, Yang K, Corre C*: Overproduction and identification of butyrolactones SCB1-8 in the antibiotic production superhost Streptomyces M1152. Org Biomol Chem 2016, 14(27):6390-6393.

Jiang T#, Wang W, Wu X, Wu W, Bai H, Ma Z, Shen Y, Yang K*, Li M*: Discovery of New Substrates for LuxAB Bacterial Bioluminescence. Chem Biol Drug Des 2016, 88(2):197-208.

2015

Li S#, Wang J#, Li X, Yin S, Wang W*, Yang K*: Genome-wide identification and evaluation of constitutive promoters in streptomycetes. Microb Cell Fact 2015, 14(1):172.

Li S#, Wang W#, Li X, Fan K, Yang K*: Genome-wide identification and characterization of reference genes with different transcript abundances for Streptomyces coelicolor. Sci Rep 2015, 5:15840.

Li X#, Wang J, Li S, Ji J, Wang W*, Yang K*: ScbR- and ScbR2-mediated signal transduction networks coordinate complex physiological responses in Streptomyces coelicolor. Sci Rep 2015, 5:14831.

Wang W#, Li X#, Li Y, Li S, Fan K, Yang K*: A genetic biosensor for identification of transcriptional repressors of target promoters. Sci Rep 2015, 5:15887.

Yin S#, Wang W#, Wang X, Zhu Y, Jia X, Li S, Yuan F, Zhang Y*, Yang K*: Identification of a cluster-situated activator of oxytetracycline biosynthesis and manipulation of its expression for improved oxytetracycline production in Streptomyces rimosus. Microb Cell Fact 2015, 14(1):46.

2014

Wang W#, Ji J#, Li X, Wang J, Li S, Pan G, Fan K, Yang K*: Angucyclines as signals modulate the behaviors of Streptomyces coelicolor. Proc Natl Acad Sci U S A 2014, 111(15):5688-5693.

2013

Wang W#, Li X#, Wang J, Xiang S, Feng X, Yang K*: An engineered strong promoter for streptomycetes. Appl Environ Microbiol 2013, 79(14):4484-4492.

Liu Z#, Wang W, Zhu Y, Gong Q, Yu W*, Lu X*: Antibiotics at subinhibitory concentrations improve the quorum sensing behavior of Chromobacterium violaceum. FEMS Microbiol Lett 2013, 341(1):37-44.

2012

Fan K#, Pan G#, Peng X, Zheng J, Gao W, Wang J, Wang W, Li Y, Yang K*: Identification of JadG as the B ring opening oxygenase catalyzing the oxidative C-C bond cleavage reaction in jadomycin biosynthesis. Chem Biol 2012, 19(11):1381-1390.

2011

Wang J#, Wang W#, Wang L, Zhang G, Fan K, Tan H, Yang K*: A novel role of 'pseudo'gamma-butyrolactone receptors in controlling gamma-butyrolactone biosynthesis in Streptomyces. Mol Microbiol 2011, 82(1):236-250.