Dr. Wen got his BSc and MSc in Nankai University. He received his Ph. D in Nankai University in 2002. From 2004 to 2005, he was a post-doctoral researcher in Centre National de la Recherche Scientifique (CNRS UPR9073), Paris, where he identified the novel exonucleolytic maturation of CCA-containing tRNA in Bacillus subtilis firstly. He joined the Institute of Microbiology, CAS as a Principal Investigator in 2005. He focuses on the systems biology and metabolic engineering for amino acids, nucleosides and proteases production in industrial microbes using novel classification and genetic manipulation tools.

Our group develops novel systematic classification and genetic manipulation tools for microbes, with which we focus on the synthesis and regulation mechanism of amino acids, nucleosides and proteases. Systems engineered strains were constructed and the metabolic fluxes in the bacteria were optimized for the maximum production of end-products. Specific interests are:
1.Rapid discrimination of newly isolated Bacillales with industrial applications using Raman spectroscopy and Screening for the stable alkaline protease from isolated wild-type strains and metagenomic libraries. Reveling the mechanism of high activity and stability of alkaline proteases in a structural perspective.
2.Genetic engineering of industrial microbes using novel genetic manipulation toolbox, including transformation methods by combining cell-wall weakening and cell-membrane fluidity disturbing, overcoming the restriction barrier of bacteria harboring multiple restriction-modification systems with the mimicking-of-DNA-methylation-patterns (MoDMP) pipeline, scarless gene knockout and chromosomal site-directed mutagenesis by combining temperature sensitive plasmids and toxic counter-selection markers.
3.Using Corynebacterium glutamicum as a model, study the mechanism of regulation in amino acids biosynthesis pathway and transportation machinery of amino acids. Construct rational designed amino acids-producing strains with systems engineering.
4.With the knowledge of metabolic flux distribution within cells, optimize the downstream fermentation process of amino acids, nucleosides and proteases production, and specifically, developed a two-stage feeding strategy based on the kind and level of feeding nutrients for improving production of L-threonine by Escherichia coli.

2004 Science and Technonlogy First Award of Chinese Society of Traditional Chinese Medicine
2003 Science and Technonlogy Second Award of Chinese Society of Traditional Chinese Medicine
2002 Tianjin Scientific and Technological Progress Second Award
2000 Tianjin Scientific and Technological Progress Second Award
1997 Tianjin Scientific and Technological Progress Second Award

[1] Guoqiang Zhang, Wenzhao Wang, Aihua Deng, Zhaopeng Sun, Yun Zhang, Yong Liang, Yongsheng Che, Tingyi Wen*. A Mimicking-of-DNA-methylation-patterns Pipeline for Overcoming the Restriction Barrier of Bacteria. PLoS Genetics. 2012, 8(9): e1002987.
[2] Shuwen Liu, Yong Liang , Qian Liu, Tongtong Tao, Shujuan Lai, Ning Chen, Tingyi Wen*. Development of a two-stage feeding strategy based on the kind and level of feeding nutrients for improving fed-batch production of L-threonine by Escherichia coli. Applied Microbiology and Biotechnology. 2012, doi:10.1007/s00253-012-4317-4.
[3] Yun Zhang, Xiuling Shang, Shujuan Lai, Guoqiang Zhang, Yong Liang, Tingyi Wen*. Development and application of an arabinose-inducible expression system by facilitating inducer uptake in Corynebacterium glutamicum. Applied and Environmental Microbiology. 2012, 78(16):5831-5838.
[4] Aaihua. Deng, Zhaopeng. Sun, Guoqiang. Zhang, Jie Wu, Tingyi Wen*. Rapid discrimination of newly isolated Bacillales with industrial applications using Raman spectroscopy. Laser Physics Letters. 2012, 9(9), 636–642.
[5] LAI ShuJuan, ZHANG Yun, LIU ShuWen, LIANG Yong, SHANG XiuLing, CHAI Xin, WEN TingYi*. Metabolic engineering and flux analysis of Corynebacterium glutamicum for L-serine production. SCIENCE CHINA Life Sciences, 2012, 55(4): 283–290.(Cover article)
[6] Yun Zhang, Xiuling Shang, Aihua Deng, Xin Chai, Shujuan Lai, Guoqiang Zhang, Tingyi Wen*. Genetic and biochemical characterization of Corynebacterium glutamicum ATP phosphoribosyltransferase and its three mutants resistant to feedback inhibition by histidine. Biochimie. 2012, 94(3):829-38.
[7] Guoqiang Zhang, Aihua Deng, Qingyang Xu, Yong Liang, Ning Chen*, Tingyi Wen*. Complete genome sequence of Bacillus amyloliquefaciens TA208, a strain for industrial production of guanosine and ribavirin. Journal of Bacteriolology. 2011, 193(12):3142-3143.
[8] Haojian Li, Guoqiang Zhang, Aihua Deng, Ning Chen, Tingyi Wen*. De novo engineering and metabolic flux analysis of Bacillus subtilis for inosine biosynthesis. Biotechnology letters. 2011, 33:1575-1580.
[9] Aihua Deng, Jie Wu, Guoqiang Zhang, Tingyi Wen*. Molecular and structural characterization of a surfactant-stable high-alkaline protease AprB with a novel structural feature unique to subtilisin family. Biochimie, 2011, 93(4):783-791.
[10] Guoqiang Zhang, Peng Bao, Yun Zhang, Aihua Deng, Ning Chen, Tingyi Wen*. Enhancing electro-transformation competency of recalcitrant Bacillus amyloliquefaciens by combining cell-wall weakening and cell-membrane fluidity disturbing. Analytical Biochemistry, 2011, 409:130–137.
[11] Aihua Deng, Jie Wu, Yun Zhang, Guoqiang Zhang, Tingyi Wen*. Purification and characterization of a surfactant-stable high-alkaline protease from Bacillus sp. B001. Bioresource Technology, 2010, 101:7100-7106.
[12] Ning Chen, Jin Huang, Zhibin Feng, Lei Yu, Qingyang Xu, Tingyi Wen*. Optimization of fermentation conditions for the biosynthesis of L-threonine by Escherichia coli. Applied Biochemistry and Biotechnolology, 2009, 158:595-604.
[13] Nathalie Mathy, Lionel Benard, Olivier Pellegrini, Roula Daou, Tingyi Wen, Ciarán Condon. 5’ to 3’ Exoribonuclease Activity in Bacteria: Role of RNase J1 in rRNA Maturation and 5’ Stability of mRNA. Cell, 2007, 129(4):681-692.
[14] Robert A. Britton, Tingyi Wen, Laura Schaefer,Oliver Pellegrini, William C. Uicker, Nathalie Mathy, Crystal Tobin, Roula Daou,Yacek Szyk ,Ciarán Condon. Maturation of the 5’ end of Bacillus subtilis 16S rRNA by the essential ribonuclease YkqC/RNase J1. Molecular Microbiology, 2007, 63 (1):127-138.
[15] Tingyi Wen, Irina A. Oussenko, Olivier Pellegrini, David H. Bechhofer, Ciarán Condon. Ribonuclease PH plays a major role in the exonucleolytic maturation of CCA-containing tRNA precursors in B. subtilis. Nucleic Acid Research, 2005, 33(11):3636-3643.