Principal Investigators
State Key Laboratory of Microbial Resources
State Key Laboratory of Mycology
CAS Key laboratory of Microbial Physiological and Metabolic Engineering
State Key Laboratory of Plant Genomics(Co-established with Institute of Genetics and Developmental Biology, CAS)
CAS Key laboratory of Pathogenic Microbiology & Immunology
The Center for Microbial Resource and Big Data-
CAS Key laboratory of Microbial Physiological and Metabolic Engineering
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Principal Investigator,Professor :

WANG Luoyi
Address：NO.1 West Beichen Road, Chaoyang District, Beijing 100101, China
Telephone/fax：010-64807034
E-mail：wangluoyi@im.ac.cn
Research Interests
Natural products have been and continue to be rich sources for new drug discovery, and the enzymatic machinery for natural products biosynthesis provides excellent opportunities for novel biocatalysts development. Our research mainly focuses on natural product biosynthesis and enzyme engineering. With recent advances in cutting-edge molecular genetic engineering and synthetic biotechnologies, we aim to:
(1) Answer pressing, unresolved fundamental questions regarding the enzymology involved in natural product biosynthetic processes;
(2) Undertake engineering of these enzymes and harness their biosynthetic ingenuity as novel biocatalysts with wide-ranging (bio)synthetic utility;
(3) Establish microbial cell factories for green and efficient production of medicinally important compounds.
Education/degrees
2008.9-2013.7 Ph.D in Medicinal Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
2004.9-2008.7 BSc in Chinese Materia Medica, Southern Medical University
Work experience
2014.11-2020.10 BrisSynBio Postdoctoral Research Associate (with Prof. Chris Willis), School of Chemistry, University of Bristol
2014.3-2014.10 Postdoctoral Researcher (with Prof. W. James Harper), Department of Food Science and Technology, The Ohio State University
2013.9-2014.3 Postdoctoral Researcher (with Prof. Jixun Zhan), Department of Biological Engineering, Utah State University
The main research areas
Natural product biosynthesis, enzyme engineering

Publication

1. Wang L, Song Z, Race PR, Spencer J, Simpson TJ, Crump MP, Willis CL. Mixing and matching genes of marine and terrestrial origin in the biosynthesis of the mupirocin antibiotics. Chem. Sci., 2020. 11. 5221-5226.

2. Walker PD, Rowe MT, Winter AJ, Weir ANM, Akter N, Wang L, Race PR, Williams C, Song Z, Simpson TJ, Willis CL, Crump MP. A Priming Cassette Generates Hydroxylated Acyl Starter Units in Mupirocin and Thiomarinol Biosynthesis. ACS Chem. Biol., 2020. 15. 494-503.

3. Walker PD, Williams C, Weir ANM, Wang L, Crosby J, Race PR, Simpson TJ, Willis CL, Crump MP. Control of β‐Branching in Kalimantacin Biosynthesis: Application of 13C NMR to Polyketide Programming. Angew. Chem. Int. Ed., 2019. 58. 12446-12450.

4. Wang L, Parnell A, Williams C, Bakar, NA, Challand MR, van der Kamp MW, Simpson TJ, Race PR, Crump MP, Willis CL. A Rieske oxygenase/epoxide hydrolase-catalysed reaction cascade creates oxygen heterocycles in mupirocin biosynthesis. Nat. Catal., 2018. 1. 968-976.

5. Thistlethwaite IRG, Bull FM, Cui C, Walker PD, Gao SS, Wang L, Song Z, Masschelein J, Lavigne R, Crump MP, Race PR, Simpson TJ, Willis CL. Elucidation of the relative and absolute stereochemistry of the kalimantacin/batumin antibiotics. Chem. Sci., 2017. 8. 6196-6201.

6. Gao SS#, Wang L#, Song Z, Hothersall J, Stevens ER, Connolly J, Winn PJ, Cox RJ, Crump MP, Race PR, Thomas CM, Simpson TJ, Willis CL. Selected Mutations Reveal New Intermediates in the Biosynthesis of Mupirocin and the Thiomarinol Antibiotics. Angew. Chem. Int. Ed., 2017. 56. 3930-3934. (VIP paper, back cover, #Equal contribution)

7. Wang L#, Qin JJ#, Chen ZH#, Zhou Y, Tang W, Zuo JP, Zhao WM. Absolute Configuration of Periplosides C and F and Isolation of Minor Spiro-orthoester Group-Containing Pregnane-type Steroidal Glycosides from Periploca sepium and Their T-Lymphocyte Proliferation Inhibitory Activities. J. Nat. Prod., 2017. 80. 1102-1109. (#Equal contribution)

8. Xu YQ, Zhou T, Zhang SW, Espinosa-Artiles P, Wang L, Zhang W, Lin M, Gunatilaka AAL, Zhan JX, Molnar I. Diversity-oriented combinatorial biosynthesis of benzenediol lactone scaffolds by subunit shuffling of fungal polyketide synthases. Proc. Natl. Acad. Sci. USA, 2014. 111. 12354-12359.

9. Wang L#, Wu J#, Yang Z#, Wang XJ, Fu Y, Liu SZ, Wang HM, Zhu WL, Zhang HY, Zhao WM. (M)- and (P)-bicelaphanol A, dimeric trinorditerpenes with promising neuroprotective activity from Celastrus orbiculatus. J. Nat. Prod., 2013. 76. 745-749. (#Equal contribution)

10. Wang L, Chen ZH, Zhou Y, Tang W, Zuo JP, Zhao WM. Structural revision of periplocosides and periperoxides, natural immunosuppressive agents from the genus Periploca. Phytochemistry, 2011. 72. 2230-2236.