Principal Investigator,Professor : Molecular Enzymology Research Group
The research works of this group is focused on some industrial enzyme from various microorganisms. Molecular technology had been used to get some enzyme gene from metagenomic liberary of extreme environment. Some importment enzyme gene had been found and been over-expressed for industrial application .
Key words: Xylanase,Cellulase,Metagenomic liberary
Background and Significance:
1) Cellulose-degrading enzymes research for biofuel production and feed.
2)Construction of metagenomic library from extreme environments and Screening for industrial enzyme genes.
3) high-efficient enzyme gene secretion expression system construction
4) Higher industrial enzyme productive engineering strain construction and
5) Study on the function and structure of archaeal chaperonin from Acidianus Tengchongeneses sp.S5
In the past year, the research works of this group has been supported by the fund of NSFC, “973” project from MOST, China , “863” project from MOST, China. Some important industry enzymes such as Xylanase，cellulase had been producted with over-expression recombinant strain and applied in feed, paper industry. A series of extreme environment metagenomic library including a rumen metagenomic BAC library had been constructed, a series of new gene of enzyme had been found in those library with HTS. A thermostability new enzyme gene had been screened by HTS. Some new archaeal chaperonin had been found in Acidianus Tengchongeneses sp.S5.A pilot plant for industial enzyme had been set up for production of xylanase, cellulase. Some enzymes had been applied successful in pulp manufacture and fuel enthanol production.