DU Wenbin----微生物研究所

State Key Laboratory of Microbial Resources

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Principal Investigator,Professor :

DU Wenbin

Address：Institute of Microbiology Chinese Academy of Science No.7 Beitucheng West Road, Room G-303 Chaoyang District, Beijing 100029 China

Telephone/fax： 010-82994195

E-mail：wenbin@im.ac.cn

Research Interests

Our research spans a broad range of Microbiology, Biochemistry, and Microfluidics. We use microfluidic droplets for high-throughput culturing and screening of single cells in nanoliter to picoliter volumes. We are developing automated workflows and devices for large scale single-cell isolation, cultivation, interaction and sequencing. We want to provide better approaches to improve the cultivation of microbial samples, to isolate and study rare microbes and extremophiles from the environments, to study the diversity, functions and evolution of complex microbial communities, and to advance clinical pathogen diagnosis and biosafety testing. Our current ongoing projects include:
1. Single-cell isolation, cultivation and sorting using high-throughput droplet microfluidic technologies.
2. Single cell whole genome amplification of uncultured microorganisms.
3. Digial Nucleic Acid Amplification and applications.
4. Isolation, identification and antimicrobial susceptibility testing of pathogens in clinical and environmental samples.

Education/degrees

06/2002 B. Sc. in Chemistry (Zhejiang University, China)
09/2002-06/2007Ph. D. in Chemistry (Zhejiang University, China) with Prof. Zhaolun Fang and Prof. Qun Fang

Work experience

09/2007-08/2011 Postdoctoral Fellow (The University of Chicago) with Prof. Rustem F. Ismagilov
09/2011-03/2013 Principle Investigator, Renmin University of China, Department of Chemistry
03/2013-Present Professor, State Key Laboratory of Microbial Resources, The Institute of Microbiology, Chinese Academy of Sciences
6/2016- Present Professor, Savaid Medical School, University of the Chinese Academy of Sciences,

The main research areas

Microfluidics and Analytical Microbiology Applications

Publication

1. Yi, Q.L.#; Cai, D.Y.#; Xiao, M.; Nie, M.Y.; Cui, Q.N.; Cheng, J.W.; Li, C.M.; Feng, J.; Urban, G.; Xu, Y.-C.*; Lan, Y.*; Du, W.B.* Direct Antimicrobial Susceptibility Testing of Bloodstream Infection on SlipChip, Biosensors & Bioelectronics, 2019, 135, 200-207.

2. Nie, M.Y.; Zheng, M.; Li, C.M.; Shen, F.; Liu, M.H.; Luo, H.B.; Song, X.H.; Lan, Y.*; Pan, J.Z.*; Du, W.B.* Assembled Step Emulsification Device for Multiplex Droplet Digital PCR, Analytical Chemistry, 2019, 91, 1779–1784.

3. Chen, D.W.; Liu, S.-J.; Du, W.B.* Chemotactic screening of imidazolinone-degrading bacteria by microfluidic SlipChip, Journal of Hazardous Materials, 2019, 366, 512-519.

4. Jin, Z.J.; Nie, M.Y.; Zhao, T.H.; Xu, J.Y.; Chen, D.W.; Yun, J.L.; Hu, R.; Ma, L.Z.; Du, W.B.* Dynamic Sessile-droplet Habitats for Controllable Cultivation of Bacterial Biofilm, Small, 2018, 14, 1800658.PDF

5. Qiao, Y.X.; Zhao, X.Y.; Zhu, J.; Tu, R.; Dong, L.B.; Wang, L.; Dong, Z.Y.; Wang, Q.H.; Du, W.B.* Fluorescence-Activated Droplet Sorting of Lipolytic Microorganisms Using a Compact Optical System, Lab On a Chip, 2018, 18, 190-196.

6. Liao, S.L; Tao, X.L.; Ju, Y.J.; Feng, J.*; Du, W.B.*; Wang, Y.P.* Multi-Channel Dynamic Interfacial Printing: An Alternative Multi-Component Droplet Generation Technique for Lab in a Drop, ACS Applied Materials and Interfaces, 2017, 9, 4345-4352.

7. Hu, Y.#; Xu, P.#; Luo, J.; He, H.X.*; Du, W.B.* Absolute Quantification of H5-subtype Avian Influenza Viruses Using Droplet Digital Loop-mediated Isothermal Amplification, Analytical Chemistry, 2017, 89 (1), 745-750. (#Equal contribution).

8. Xu, P.; Zheng, X.; Tao, Y.; Du, W.B.* Cross-Interface Emulsification for Generating Size-Tuneable Droplets, Analytical Chemistry, 2016, 88, 3171-3177.

9. Jiang, C.Y.; Dong, L.B.; Zhao, J.K.; Hu, X.F.; Shen, C.H.; Qiao, Y.X.; Zhang, X.Y.; Wang, Y.P.; Ismagilov, R.F.; Liu, S.J.*; Du, W.B.* High throughput Single-cell Cultivation on Microfluidic Streak Plates, Applied Environmental Microbiology, 2016, 82, 2210-2218.

10. Liu, X.; Yi, Q.L.; Han, Y.Z.; Liang, Z.N.; Shen, C.H.; Zhou, Z.Y.; Sun J.L.; Li, Y.Z.; Du, W.B.*; Cao, R.* A Robust Microfluidic Device for Synthesis and Crystal Growth of Organometallic Polymers with Highly Organized Structures, Angewandte Chemie International Edition, 2015, 54, 1846-1850.

11. Cai, D.Y.; Xiao, M.; Xu, P.; Xu, Y.C.*; Du, W.B.* An Integrated Microfluidic Device Utilizing Dielectrophoresis and Multiplex Array PCR for Point-of-Care Detection of Pathogens, Lab On a Chip, 2014, 14, 3917-3924. (HOT Article, Front Cover)

12. Shen, C.H.; Xu, P.; Huang, Z.; Liu, S.J.*; Du, W.B.* Bacterial Chemotaxis on SlipChip, Lab On a Chip, 2014, 14, 3074-3080.

13. Wang, S.J.; Chen, S.M.; Wang, J.N.; Xu, P.; Luo, Y.M.; Nie, Z.X.*; Du, W. B.* Interface Solution Isoelectric Focusing with in situ MALDI-TOF Mass Spectrometry, Electrophoresis, 2014, 35, 2528-2533.

14. Du, W.B.#; Sun, M.#; Gu, S.Q.#; Zhu, Y.; Fang, Q.* Automated Microfluidic Screening Assay Platform Based on DropLab, Analytical Chemistry, 2010, 82, 9941-9947 (#Equal contribution).

15. Li, L.; Du, W.B.*; Ismagilov, R.F.* Multiparameter Screening on SlipChip Used for Nanoliter Protein Crystallization Combining Free Interface Diffusion and Microbatch Methods, Journal of the American Chemical Society, 2010, 132, 112-119.

16. Shen, F.; Du, W.B.; Kreutz, J.E.; Fok, A.; Ismagilov, R.F.* Digital PCR on a SlipChip, Lab on a Chip, 2010, 10, 2666-2672.

17. Du, W.B.; Li, L.; Nichols, K.P.; Ismagilov, R.F.* SlipChip, Lab on a Chip, 2009, 9, 2286-2292.

18. Chen, D.L.#; Du, W.B.#; Liu, Y.#; Liu, W.S.#; Kuznetsov, A.; Mendez, F.E.; Philipson, L.H.; Ismagilov, R.F.* The chemistrode: A Droplet-based Microfluidic Device for Stimulation and Recording with High Temporal, Spatial, and Chemical Resolution, Proceedings of the National Academy of Sciences of the United States of America, 2008, 105, 16843-16848 (#Equal contribution).

19. Du, W.B.; Fang, Q.*; Fang, Z.L. Microfluidic Sequential Injection Analysis in a Short Capillary, Analytical Chemistry, 2006, 78, 6404-6410.

20. Du, W.B.; Fang, Q.*; He, Q.H.; Fang, Z.L. High-throughput Nanoliter Sample Introduction Microfluidic Chip-based Flow Injection Analysis System with Gravity-driven Flows, Analytical Chemistry, 2005, 77, 1330-1337.