Principal Investigator,Professor : Molecular Mechanism of RNA Silence and Disease Resistance in Plant

GUO Huishan
Dept:State Key Laboratory of Plant Genomics 
Address:NO.1 West Beichen Road, Chaoyang District, Beijing 100101, China
Telephone/fax:+86 (0) 10-64847989
Research Interests
Her research group mainly focuses on RNA silencing and sRNA regulated pathways during plant-microbe interactions, for the purpose of dissecting mechanisms of plant resistance and susceptibility to virus well as fungi. The ongoing projects mainly use Arabidopsis and Nicotiana as model plants for study but all the results aim to apply for major crops such as cotton and potato.
BSc Sun Yat-sen (Zhongshan) University, China
PhD Centro Nacional de Biotecnologia CSIC, Universidad Autonoma de Madrid, Spain
Work experience
Teaching Assistant, Center of Biotechnology, Zhongshan University, China
Guest Investigator, Centro de Biologia Molecular "Severo Ochoa", Universidad Autonoma de Madrid, Spain
Postdoctoral Fellow, Centro Nacional de Biotecnologia CSIC. Universidad Autonoma de Madrid, Spain
Research Fellow, Institute of Molecular Agrobiology, National University of Singapore
Research Fellow, Temasek Life Sciences Laboratory, National University of Singapore
Acting Principal Investigator, Temasek Life Sciences Laboratory, Singapore
Principle Investigator, the “Hundred Talents” Program of Chinese Academy of Sciences, Institute of Microbiology, CAS, China
Member of the Academic Committee of IMCAS
Deputy Director of the 4th Academic Committee of State Key laboratory of Plant Genomics
Member of the 8th Academic Committee of IMCAS (2008-2012)
Member of Academic ethics and equity committee of IMCAS (2008-2012)
Member of the 3th Academic Committee of State Key laboratory of Plant Genomics (2011-2015)
Member of the 9th Council of Chinese Society of Plant Pathology (2010-2014)
Member of the 1th Academic Committee of Key Laboratory of Tropical Crops Germplasm Resources Utilization (2011-2015)
MPP Editorial Board 2018-
PBJ Editorial Board 2018-
PloS Pathogens Associate Editor 2014-
PloS Pathogens Guest Editor 2013-2014
BMC Genomics Associate Editor 2014-2018
MPP Associate Editor 2012-2017
MPMI Associate Editor 2013-2015
Received “Outstanding Youth Scientist Award (National Science Foundation of China)”
Special government allowances of the State Council
Special Professor of University of Chinese Academy of Sciences
Special Key Talents of Chinese Academy of Sciences
Special Core Talents of Chinese Academy of Sciences
1. Cui, C.#, Wang, J.J.#, Zhao, J.H.#, Fang, Y.Y, He, X.F., Guo, H.S.*, Duan, C.G.*(2020). A Brassica miRNA Regulates Plant Growth and Immunity through Distinct Modes of Action. Molecular Plant 13(2): 231-245.
2.Gao, F.#, Zhang, B.S.#, Zhao, J.H., Huang, J.F., Jia, P.S., Wang, S., Zhang, J., Zhou, J.M., Guo, H.S.*(2019)Deacetylation of chitin oligomers increases virulence in soil-borne fungal pathogens. Nature Plants 5(11): 1167-1176.
3. Zhao, J. H., Guo, H.S.*(2019).Trans-kingdom RNA interactions drive the evolutionary arms race between hosts and pathogens. Curr Opin Genet Dev 58-59:62-69.
4. Liu, X.L., Zhao, J.H., Guo, H.S.*(2019).IBM1-dependent H3K9 demethylation enables self-silencing of an exogenous silencer for the non-cell autonomous silencing of an endogenous target gene. J Genet Genomics 46(3):149-153.
5.Jin, Y., Zhao, J. H., Zhao, P., Zhang, T., Wang, S., Guo, H.S.*. (2019)A fungal milRNA mediates epigenetic repression of a virulence gene in Verticillium dahliae. Philos Trans R Soc Lond B Biol Sci 374(1767):20180309.
6.Zhao, J. H., Liu, X. L., Fang, Y. Y., Fang, R. X., Guo, H.S.*.(2018)CMV2b-Dependent Regulation of Host Defense Pathways in the Context of Viral Infection. Viruses 10(11).
7.Qin, J., Wang, K., Sun, L., Xing, H., Wang, S., Li, L., Chen, S., Guo, H.S.*, and Zhang, J.* (2018). The plant specific transcription factors CBP60g and SARD1 are targeted by a Verticillium secretory protein VdSCP41 to modulate immunity. eLife 7.
8.Jin, Y.#, Zhao, P.#, Fang, Y.Y., Gao, F., Guo, H.S.*, and Zhao, J.H.* (2018). Genome-wide profiling of sRNAs in the Verticillium dahliae-infected Arabidopsis roots. Mycology, 1-11.
9.Hua,C.L.#, Zhao, J.H.#, and Guo, H.S.* (2018). Trans-kingdom RNA silencing in Plant–Fungal Pathogen Interactions. Mol Plant 11, 235-244.
10.Du, X., Wang, S., Gao, F., Zhang, L., Zhao, J.H., Guo, H.S.*, and Hua, C.L.* (2017). Expression of pathogenesis-related genes in cotton roots in response to Verticillium dahliae PAMP molecules. Science China. Life sciences 60, 852-860.
11. Jin, Y., Zhang, T., and Guo, H.S.* (2017). Application of host induced gene silencing in crop protection against fungal diseases. Sheng wu gong cheng xue bao = Chinese journal of biotechnology 33, 161-169.
12.Zhang, L.S.#, Ni, H.#, Du, X., Wang, S., Ma, X.W., Nurnberger, T., Guo, H.S.*, and Hua, C.L.* (2017). The Verticillium-specific protein VdSCP7 localizes to the plant nucleus and modulates immunity to fungal infections. The New phytologist 215, 368-381.
13.Zhao, Y.L.*, Zhang, T., and Guo, H.S. (2017). Penetration Assays, Fungal Recovery and Pathogenicity Assays for Verticillium dahliae. Bio-Protocol 7.
14.Zhou, T.T., Zhao, Y.L., and Guo, H.S.* (2017). Secretory proteins are delivered to the septin-organized penetration interface during root infection by Verticillium dahliae. PLoS pathogens 13, e1006275.
15.Fang, Y.Y.#, Zhao, J.H.#, Liu, S.W., Wang, S., Duan, C.G., and Guo, H.S.* (2016). CMV2b-AGO Interaction Is Required for the Suppression of RDR-Dependent Antiviral Silencing in Arabidopsis. Frontiers in Microbiology 7, 1329.
16.Lv, D.Q.#, Liu, S.W.#, Zhao, J.H., Zhou, B.J., Wang, S.P., Guo, H.S., and Fang, Y.Y. * (2016). Replication of a pathogenic non-coding RNA increases DNA methylation in plants associated with a bromodomain-containing viroid-binding protein. Scientific reports 6, 35751.
17.Wang, S., Xing, H., Hua, C., Guo, H.S.*, and Zhang, J.* (2016). An improved single-step cloning strategy simplifies the Agrobacterium tumefaciens-mediated transformation (ATMT)-based gene disruption method in Verticillium dahliae. Phytopathology.
18.Zhang, T., Jin, Y., Zhao, J.H., Gao, F., Zhou, B.J., Fang, Y.Y., and Guo, H.S.* (2016). Host-Induced Gene Silencing of the Target Gene in Fungal Cells Confers Effective Resistance to the Cotton Wilt Disease Pathogen Verticillium dahliae. Mol Plant 9, 939-942.
19.Zhang, T.#, Zhao, Y.L.#, Zhao, J.H.#, Wang, S., Jin, Y., Chen, Z.Q., Fang, Y.Y., Hua, C.L., Ding, S.W., and Guo, H.S.* (2016). Cotton plants export microRNAs to inhibit virulence gene expression in a fungal pathogen. Nature plants 2, 16153.
20.Zhao, J.H.*, Fang, Y.Y., Duan, C.G., Fang, R.X., Ding, S.W., and Guo, H.S.* (2016). Genome-wide identification of endogenous RNA-directed DNA methylation loci associated with abundant 21-nucleotide siRNAs in Arabidopsis. Scientific reports 6, 36247.
21.Zhao, J.H., Hua, C.L., Fang, Y.Y., and Guo, H.S.* (2016). The dual edge of RNA silencing suppressors in the virus-host interactions. Current opinion in virology 17, 39-44.
22.Zhao, Y.L., Zhou, T.T., and Guo, H.S.* (2016). Hyphopodium-Specific VdNoxB/VdPls1-Dependent ROS-Ca2+ Signaling Is Required for Plant Infection by Verticillium dahliae. PLoS pathogens 12, e1005793.
23.Fang, Y.Y., Smith, N.A., Zhao, J.H., Lee, J.R., Guo, H.S., and Wang, M.B.* (2015). Cloning and profiling of small RNAs from cucumber mosaic virus satellite RNA. Methods in molecular biology 1236, 99-109.
24.Jin, Y., and Guo, H.S.* (2015). Transgene-induced gene silencing in plants. Methods Mol Biol 105-117.
25.Shen, W.X., Au, P.C., Shi, B.J., Smith, N.A., Dennis, E.S., Guo, H.S., Zhou, C.Y., and Wang, M.B.* (2015). Satellite RNAs interfere with the function of viral RNA silencing suppressors. Front Plant Sci 281.
26.Wang, J.J., Zhang, L., and Guo, H.S.* (2015). Arabidopsis Leaf Explant Culture. Bio-Protocol 5(22).
27.Wang, J.J., and Guo, H.S.* (2015). Cleavage of INDOLE-3-ACETIC ACID INDUCIBLE28 mRNA by microRNA847 upregulates auxin signaling to modulate cell proliferation and lateral organ growth in Arabidopsis. The Plant Cell 574-590.
28. Zahid, K.#, Zhao, J.H.#, Smith, N.A., Schumann, U., Fang, Y.Y., Dennis, E.S., Zhang, R., Guo, H.S.*, and Wang, M.B.* (2015). Nicotiana small RNA sequences support a host genome origin of cucumber mosaic virus satellite RNA. PLoS genetics 11, e1004906.
29.Zhao, P., Zhao, Y.L., Jin, Y., Zhang, T., and Guo, H.S.* (2014). Colonization process of Arabidopsis thaliana roots by a green fluorescent protein-tagged isolate of Verticillium dahliae. Protein & cell 5, 94-98.
30. Feng, L.#, Duan, C.G.#, and Guo, H.S.* (2013). Inhibition of in vivo Slicer activity of Argonaute protein 1 by the viral 2b protein independent of its dsRNA-binding function. Molecular plant pathology 14, 617-622.
31.Yang, L.P., Fang, Y.Y., An, C.P., Dong, L., Zhang, Z.H., Chen, H., Xie, Q., and Guo, H.S.* (2013). C2-mediated decrease in DNA methylation, accumulation of siRNAs, and increase in expression for genes involved in defense pathways in plants infected with beet severe curly top virus. The Plant journal 73, 910-917.
32.Duan, C.G.#, Fang, Y.Y.#, Zhou, B.J., Zhao, J.H., Hou, W.N., Zhu, H., Ding, S.W., and Guo, H.S.* (2012). Suppression of Arabidopsis ARGONAUTE1-mediated slicing, transgene-induced RNA silencing, and DNA methylation by distinct domains of the Cucumber mosaic virus 2b protein. The Plant cell 24, 259-274.
33. Duan, C.G., Wang, C.H., and Guo, H.S.* (2012). Application of RNA silencing to plant disease resistance. Silence 3, 5.
34. Xu, N., Li, Y., Zhao, Y.T., Guo, L., Fang, Y.Y., Zhao, J.H., Wang, X.J., Huang, L., and Guo, H.S.* (2012). Identification and characterization of small RNAs in the hyperthermophilic archaeon Sulfolobus solfataricus. PloS one 7, e35306.
35.Zhou, B.J., Jia, P.S., Gao, F., and Guo, H.S.* (2012). Molecular characterization and functional analysis of a necrosis- and ethylene-inducing, protein-encoding gene family from Verticillium dahliae. Molecular plant-microbe interactions : MPMI 25, 964-975.
36.Zhu, H., and Guo, H.S.* (2012). The role of virus-derived small interfering RNAs in RNA silencing in plants. Science China. Life sciences 55, 119-125.
37.Dong, L., Liu, M., Fang, Y.Y., Zhao, J.H., He, X.F., Ying, X.B., Zhang, Y.Y., Xie, Q., Chua, N.H., and Guo, H.S.* (2011). DRD1-Pol V-dependent self-silencing of an exogenous silencer restricts the non-cell autonomous silencing of an endogenous target gene. The Plant journal 68, 633-645.
38.Hou, W.N., Duan, C.G., Fang, R.X., Zhou, X.Y., and Guo, H.S.* (2011). Satellite RNA reduces expression of the 2b suppressor protein resulting in the attenuation of symptoms caused by Cucumber mosaic virus infection. Molecular plant pathology 12, 595-605.
39.Zhang, Z., Chen, H., Huang, X., Xia, R., Zhao, Q., Lai, J., Teng, K., Li, Y., Liang, L., Du, Q., Zhou, X., Guo, H., and Xie, Q.* (2011). BSCTV C2 attenuates the degradation of SAMDC1 to suppress DNA methylation-mediated gene silencing in Arabidopsis. The Plant cell 23, 273-288.
40.Zhu, H., Duan, C.G., Hou, W.N., Du, Q.S., Lv, D.Q., Fang, R.X., and Guo, H.S.* (2011). Satellite RNA-derived small interfering RNA satsiR-12 targeting the 3' untranslated region of Cucumber mosaic virus triggers viral RNAs for degradation. Journal of virology 85, 13384-13397.
41. Chen, H., Zhang, Z., Teng, K., Lai, J., Zhang, Y., Huang, Y., Li, Y., Liang, L., Wang, Y., Chu, C., Guo, H., and Xie, Q.* (2010). Up-regulation of LSB1/GDU3 affects geminivirus infection by activating the salicylic acid pathway. The Plant journal 62, 12-23.
42.Gao, F.#, Zhou, B.J.#, Li, G.Y., Jia, P.S., Li, H., Zhao, Y.L., Zhao, P., Xia, G.X., and Guo, H.S.* (2010). A glutamic acid-rich protein identified in Verticillium dahliae from an insertional mutagenesis affects microsclerotial formation and pathogenicity. PloS one 5, e15319.
43.Li, Y., Liu, X., Huang, L., Guo, H.S.*, and Wang, X.J.* (2010). Potential coexistence of both bacterial and eukaryotic small RNA biogenesis and functional related protein homologs in Archaea. Journal of Genetics and Genomics 37, 493-503.
44.Liu, L.J., Zhang, Y.Y., Tang, S.Y., Zhao, Q.Z., Zhang, Z.H., Zhang, H.W., Dong, L., Guo, H.S., and Xie, Q.* (2010). An efficient system to detect protein ubiquitination by agroinfiltration in Nicotiana benthamiana. The Plant journal 61, 893-903.
45.Teng, K.L., Chen, H., Lai, J.B., Zhang, Z.H., Fang, Y.Y., Xia, R., Zhou, X.P., Guo, H.S., and Xie, Q.* (2010). Involvement of C4 protein of beet severe curly top virus (family Geminiviridae) in virus movement. PloS one 5, e11280.
46.Wang, W.Q., Wu, Y.R., Li, Y., Xie, J.Y., Zhang, Z.H., Deng, Z.Y., Zhang, Y.Y., Yang, C.P., Lai, J.B., Zhang, H.W., Bao, H.Y., Tang, S.Y., Yang, C.W., Gao, P., Xia, G.X., Guo, H.S., and Xie, Q. *(2010). A large insert Thellungiella halophila BIBAC library for genomics and identification of stress tolerance genes. Plant molecular biology 72, 91-99.
47.Ying, X.B., Dong, L., Zhu, H., Duan, C.G., Du, Q.S., Lv, D.Q., Fang, Y.Y., Garcia, J.A., Fang, R.X., and Guo, H.S.* (2010). RNA-dependent RNA polymerase 1 from Nicotiana tabacum suppresses RNA silencing and enhances viral infection in Nicotiana benthamiana. The Plant cell 22, 1358-1372.
48. Lai, J.B., Chen, H., Teng, K.L., Zhao, Q.Z., Zhang, Z.H., Li, Y., Liang, L., Xia, R., Wu, Y.R., Guo, H.S., and Xie, Q.* (2009). RKP, a RING finger E3 ligase induced by BSCTV C4 protein, affects geminivirus infection by regulation of the plant cell cycle. The Plant journal 57, 905-917.
49.Zheng, N.Y., Xia, R., Yang, C.W., Yin, B., Li, Y., Duan, C.G., Liang, L., Guo, H.S., and Xie, Q.*(2009). Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice. Vaccine 27, 5001-5007.
50.Duan, C.G., Wang, C.H., Fang, R.X., and Guo, H.S.* (2008). Artificial MicroRNAs highly accessible to targets confer efficient virus resistance in plants. Journal of virology 82, 11084-11095.
51. He, X.F., Fang, Y.Y., Feng, L., and Guo, H.S.* (2008). Characterization of conserved and novel microRNAs and their targets, including a TuMV-induced TIR–NBS–LRR class R gene-derived novel miRNA in Brassica. FEBS letters 582, 2445-2452.
52.Zhang, Y.Y., Li, Y., Gao, T., Zhu, H., Wang, D.J., Zhang, H.W., Ning, Y.S., Liu, L.J., Wu, Y.R., Chu, C.C., Guo, H.S., and Xie, Q.* (2008). Arabidopsis SDIR1 enhances drought tolerance in crop plants. Bioscience, biotechnology, and biochemistry 72, 2251-2254.
53.Du, Q.S.#, Duan, C.G.#, Zhang, Z.H.#, Fang, Y.Y., Fang, R.X., Xie, Q., and Guo, H.S.* (2007). DCL4 targets Cucumber mosaic virus satellite RNA at novel secondary structures. Journal of virology 81, 9142-9151.
54. Zhang, Y.Y., Yang, C.W., Li, Y., Zheng, N.Y., Chen, H., Zhao, Q.Z., Gao, T., Guo, H.S., and Xie, Q.* (2007). SDIR1 is a RING finger E3 ligase that positively regulates stress-responsive abscisic acid signaling in Arabidopsis. The Plant cell 19, 1912-1929.
55.Zhang, Z.H., Xie, Q., and Guo, H.S.* (2007). Antiviral defense in plants. Plant Viruses 1, 21-26.
56. Xie, Q., and Guo, H.S.* (2006). Systemic antiviral silencing in plants. Virus research 118, 1-6.
57.Guo, H.S., Xie, Q., Fei, J.F., and Chua, N.H*. (2005). MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for arabidopsis lateral root development. The Plant cell 17, 1376-1386.