DENG Tao----微生物研究所

CAS Key laboratory of Pathogenic Microbiology & Immunology

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Principal Investigator,Professor :

DENG Tao

Address：NO.1 West Beichen Road, Chaoyang District, Beijing 100101, China

Telephone/fax：010-64807980

E-mail：dengt@im.ac.cn

Research Interests

Our laboratory mainly focuses on studying the fundamental replication mechanisms of influenza virus. I am particularly interested in uncovering the regulation mechanisms of viral genome transcription/replication and their underlying structural and functional basis; elucidating the structural and functional properties of the RNA sequence and characteristics in the regulation of influenza virus RNA syntheses, gene expression, trafficking and selective packaging; identifying the virus-host interactions in the regulation of viral RNA syntheses and host specificity and revealing their underlying mechanisms. Based on the above basic research achievements, we are also interested in developing novel antiviral drugs and vaccines.

Education/degrees

09/1988-06/1992 BSc in Biological Sciences, Department of Biology, Lanzhou University, P.R. China

11/2002-03/2006 DPhil in Molecular Virology, Sir William Dunn School of Pathology, Oxford University, UK

Work experience

06/1992-08/2000 Research Assistant, Lanzhou Institute of Biological Products (LIBP), Ministry of Public Health, Lanzhou, China

04/2001-11/2001 Academic Visitor, Sir William Dunn School of Pathology, University of Oxford, UK

11/2001-03/2006 Research Assistant, Sir William Dunn School of pathology, University of Oxford. UK

04/2006-12/2008 Postdoctoral Research Scientist, Sir William Dunn School of Pathology, University of Oxford, UK

01/2009-10/2009 Senior Scientist, National Institute of Biological Standard And Control (NIBSC), London, UK

10/2009-03/2020 Primary Investigator, Institute of Pathogen Biology, Chinese Academy of Medical Science

03/2020- present Primary Investigator, Institute of Microbiology, Chinese Academy of Sciences

The main research areas

1. Molecular mechanisms of influenza virus RNA syntheses and gene expressions.
2. The role of the viral RNA structures in regulation of the virus replication.
3. The role of host factors in viral replication and host-range determinations.
4. Research and Development of influenza vaccines and antivirals.

Publication

1. Yang R, Pan M, Guo J, Huang Y, Zhang QC*, Deng T*, Wang J*. 2024. Mapping of the influenza A virus genome RNA structure and interactions reveals essential elements of viral replication. Cell Rep., 43(3):113833. doi: 10.1016/j.celrep.2024.113833.

2. Zhang L, Shao Y, Wang Y, Yang Q, Guo J, Gao GF, Deng T*. 2024. Twenty natural amino acid substitution screening at the last residue 121 of influenza A virus NS2 protein reveals the critical role of NS2 in promoting virus genome replication by coordinating with viral polymerase. J. Virol., 98(1):e0116623. doi: 10.1128/jvi.01166-23.

3. Cao M, Jia Q, Li J, Zhao L, Zhu L, Zhang Y, Li S, Deng T*. 2024. Naturally occurring PAE206K point mutation in 2009 H1N1 pandemic influenza viruses impairs viral replication at high temperatures. Virol. Sin., 39(1):71-80. doi: 10.1016/j.virs.2023.11.005.

4. Zhang L, Wang Y, Shao Y, Guo J, Gao GF*, Deng T*. Fine Regulation of Influenza Virus RNA Transcription and Replication by Stoichiometric Changes in Viral NS1 and NS2 Proteins. J. Virol., 97(5):e0033723. doi: 10.1128/jvi.00337-23.

5. Shu C, Sun Q, Fan G, Peng K, Yu Z, Luo Y, Gao S, Ma J, Deng T, Hu S, Wu L. 2024. VarEPS-Influ: an risk evaluation system of occurred and virtual variations of influenza virus genomes. Nucleic Acids. Res., 52(D1):D798-D807. doi: 10.1093/nar/gkad912.

6. Wang L, Guo J, Wang Y, Zhao P, Liu B, Zhang Y, Xiong Y, Chen Q, Lin L, Li L, He X, Tan Y, Cao M, Yi J, Deng T*, Lu C*. 2022. Anti-inflammatory effects of Chaishi Tuire Granules on influenza A treatment by mediating TRAF6/MAPK14 axis. Front. Med. (Lausanne), 9:943681. doi: 10.3389/fmed.2022.943681.

7. Pan M, Zhang W, Xiao Y, Lai Y, Cao M, Wang J, Deng T*. 2022. The Hierarchical Sequence Requirements of the H1 Subtype-Specific Noncoding Regions of Influenza A Virus. Microbiol. Spectr., 10(6):e0315322. doi: 10.1128/spectrum.03153-22.

8. Gao P, Ji M, Liu X, Chen X, Liu H, Li S, Jia B, Li C, Ren L, Zhao X, Wang Q, Bi Y, Tan X, Hou B, Zhou X, Tan W, Deng T, Wang J, Gao GF, Zhang F. 2022. Apolipoprotein E mediates cell resistance to influenza virus infection. Sci. Adv., 8(38):eabm6668. doi: 10.1126/sciadv.abm6668.

9. Zhao J, Wang J, Pang X, Liu Z, Li Q, Yi D, Zhang Y, Fang X, Zhang T, Zhou R, Zhang T, Guo Z, Liu W, Li X, Liang C, Deng T, Guo F, Yu L, Cen S. 2022. An anti-influenza A virus microbial metabolite acts by degrading viral endonuclease PA. Nat. Commun., 13(1):2079. doi: 10.1038/s41467-022-29690-x.

10. Zhou Z, Zhang X, Lei X, Xiao X, Jiao T, Ma R, Dong X, Jiang Q, Wang W, Shi Y, Zheng T, Rao J, Xiang Z, Ren L, Deng T, Jiang Z, Dou Z, Wei W, Wang J. 2021. Sensing of cytoplasmic chromatin by cGAS activates innate immune response in SARS-CoV-2 infection. Signal Transduct Target Ther., 6(1):382. doi: 10.1038/s41392-021-00800-3.

11. Xiao Y, Zhang W, Pan M, Bauer DLV, Bi Y, Cao M, Fodor E, Deng T*. 2021. Synergistic Effect between 3'-Terminal Noncoding and Adjacent Coding Regions of the Influenza A Virus Hemagglutinin Segment on Template Preference. J. Virol., 95(18):e0087821. doi: 10.1128/JVI.00878-21.

12. Gao S, Zhang W, Lu C, Cao M, Cen S, Peng Y, Deng T*. 2019. Identification of a type-specific promoter element that differentiates between influenza A and B viruses. J. Virol., 93(23). doi: 10.1128/JVI.01164-19.

13. Peng Q, Liu Y, Peng R, Wang M, Yang W, Song H, Chen Y, Liu S, Han M, Zhang X, Wang P, Yan J, Zhang B, Qi J, Deng T, Gao GF, Shi Y. 2019. Structural insight into RNA synthesis by influenza D polymerase. Nat Microbiol., 4(10):1750-1759. doi: 10.1038/s41564-019-0487-5.

14. Wang J, Zhang Y, Li Q, Zhao J, Yi D, Ding J, Zhao F, Hu S, Zhou J, Deng T, Li X, Guo F, Liang C, Cen S. 2019. Influenza Virus Exploits an Interferon-Independent lncRNA to Preserve Viral RNA Synthesis through Stabilizing Viral RNA Polymerase PB1. Cell Rep., 27(11). doi: 10.1016/j.celrep.2019.05.036.

15. Li C, Wang Z, Cao Y, Wang L, Ji J, Chen Z, Deng T, Jiang T, Cheng G, Qin FX. 2017. Screening for Novel Small-Molecule Inhibitors Targeting the Assembly of Influenza Virus Polymerase Complex by a Bimolecular Luminescence Complementation-Based Reporter System. J. Virol., 91(5).doi: 10.1128/JVI.02282-16.

16. Wang J, Li J, Zhao L, Cao M, Deng T*. 2017. Dual Roles of the Hemagglutinin Segment-Specific Noncoding Nucleotides in the Extended Duplex Region of the Influenza A Virus RNA Promoter. J. Virol., 91(1). doi: 10.1128/JVI.01931-16.

17. Wang Z, Zhao F, Gao Q, Liu Z, Zhang Y, Li X, Li Y, Ma W, Deng T, Zhang Z, Cen S. 2015. Establishment of a High-Throughput Assay to Monitor Influenza A Virus RNA Transcription and Replication. PLoS One, 10(7). doi: 10.1371/journal.pone.0133558

18. Wang J, Peng Y, Zhao L, Cao M, Hung T, Deng T*. 2014. Influenza A virus Utilizes a Suboptimal Kozak Sequence to Fine-tune Virus Replication and Host Response. J Gen Virol., 96(Pt 4). doi: 10.1099/vir.0.000030.

19. Li C, Wu A, Peng Y, Wang J, Guo Y, Chen Z, Zhang H, Wang Y, Dong J, Wang L, Qin FX, Cheng G, Deng T*, Jiang T*. 2014. Integrating computational modeling and functional assays to decipher the structure-function relationship of influenza virus PB1 protein. Sci Rep., 26(4). doi: 10.1038/srep07192.

20. Cao M, Wei C, Zhao L, Wang J, Jia Q, Wang X, Jin Q, Deng T*. 2014. DnaJA1/Hsp40 is co-opted by influenza A virus to enhance its viral RNA polymerase activity. J. Virol., 88(24), doi: 10.1128/JVI.02475-14.

21. Zhao L, Peng Y, Zhou K, Cao M, Wang J, Wang X, Jiang T,Deng T*. 2014. New insights into the nonconserved noncoding region of the subtype-determinant hemagglutinin and neuraminidase segments of influenza A viruses. J. Virol., 88(19). doi: 10.1128/JVI.01337-14.

22. Zhou Z, Cao M, Guo Y, Zhao L, Wang J, Jia X, Li J, Wang C, Gabriel G, Xue Q, Yi Y, Cui S, Jin Q*, Wang J*, Deng T*. 2014. Fragile X mental retardation protein stimulates ribonucleoprotein assembly of Influenza A virus. Nat. Commun., 5:3259. doi: 10.1038/ncomms4259.

23. Tefsen B, Lu G, Zhu Y, Haywood J, Zhao L, Deng T, Qi J, Gao GF. 2014. The N-terminal domain of PA from bat-derived influenza-like virus H17N10 has endonuclease activity. J. Virol., 88(4):1935-41. doi: 10.1128/JVI.03270-13.

24. Li C, Ba Q, Wu A, Zhang H, Deng T*, Jiang T*. 2013. A peptide derived from C-terminus of PB1 inhibits influenza virus replication by interfering with viral polymerase assembly. FEBS J., 280(4). doi: 10.1111/febs.12107.

25. Dreger M, Leung BW, Brownlee GG*, Deng T*. 2009. A quantitative proteomics strategy to detect protein regions affected by protein-protein interactions. Protein Sci., 18(7). doi: 10.1002/pro.159.

26. Kashiwagi T, Leung BW, Deng T, Chen H, Brownlee GG. 2009. The N-terminal region of the PA subunit of the RNA polymerase of influenza A/HongKong/156/97 (H5N1) influences promoter binding. PLoS One. 4(5):e5473. doi: 10.1371/journal.pone.0005473.

27. Yuan P, Bartlam M, Lou Z, Chen S, Zhou J, He X, Lv Z, Ge R, Li X, Deng T, Fodor E, Rao Z, Liu Y. 2009. Crystal structure of an avian influenza polymerase PA(N) reveals an endonuclease active site. Nature. 458(7240):909-13. doi: 10.1038/nature07720.

28. Chase G^#, Deng T^#, Fodor E, Mayer D, Schwemmle M, Brownlee G. 2006. Hsp90 inhibitors reduce influenza virus replication in cell culture. Virology, 377(2). doi: 10.1016/j.virol.2008.04.040.

29. Deng T^#, Sharps J, Brownlee GG. 2006. The role of the influenza virus heterotrimeric RNA polymerase complex in the initiation of replication. J Gen Virol., 87(Pt11): 3373-3377.

30. Deng T^#, Engelhardt OG*, Thomas B, Thomas B, Akoulitchev AV, Brownlee GG, Fodor E. 2006. The role of Ran binding protein 5 (RanBP5) in the nuclear import and assembly of he influenza virus RNA polymerase complex. J. Virol., 80:11911-11919.

31. Deng T^#, Vreede FT^#, Brownlee GG. 2006. Different de novo initiation strategies are used by influenza virus RNA polymerase on its cRNA and viral RNA promoters during viral RNA replication. J. Virol., 80(5):2337-2348.

32. Deng T^#, Sharps J, Fodor E, Brownlee GG. 2005. In vitro assembly of PB2 with PB1-PA dimer supports a new model assembly of influenza A virus polymerase subunits into a functional trimeric complex. J. Virol., 79(13):8669-8674.

33. Crow M^#, Deng T^#, Addley M, Brownlee GG. 2004. Mutational analysis of the influenza virus cRNA promoter and identification of nucleotides critical for replication. J. Virol., 78(12):6263-6270.

34. Fodor E, Mingay LJ, Crow M, Deng T, Brownlee GG. 2003. A single amino acid mutation in the PA subunit of the influenza virus RNA polymerase promotes the generation of defective interfering RNAs. J. Virol., 77(8):5017-5020.

35. Fodor E, Crow M, Mingay LJ, Deng T, Sharps J, Fechter P, Brownlee GG. 2002. A single amino acid mutation in the PA subunit of the influenza virus RNA polymerase inhibits endonucleolytic cleavage of capped RNAs. J. Virol., 76(18):8989-9001.