Principal Investigator,Professor : Protein Engineering and Microbial Enzymes Applications

Shuang-Yan Tang(Deputy Director)
Title:Professor, Ph.D.
Dept.:CAS Key Laboratory of Microbial Physiological and Metabolic Engineering
Address:NO.1 Beichen West Road, Chaoyang District,Beijing 100101, P.R.China
Ph.D. 2006 Food Science, Seoul National University
M.S. 1998 Biochemistry, Wuhan University
B.S. 1995 Biochemistry, Wuhan University
2011-present Institute of Microbiology, Chinese Academy of Sciences, Professor
2006-2010 The Pennsylvania State University, Postdoctoral researcher
1998-2003 Yunnan Provincial Institute for Drug Control
Research Interests
1. Carbohydrate enzymes engineering
We are engineering microbial enzymes for novel or improved properties using combinatorial (evolutionary) as well as rational (structure-based) protein design techniques. We are especially interested in carbohydrate enzymes such as glycosidase and glycosyltransferase. The substrate or product specificity, catalytic activity and stability of these enzymes are engineered for extensive application.
2. Biosynthesis of high value small molecules
We are engineering the biosynthesis pathways of small molecules including pharmaceuticals, food additives and other commodity chemicals in E. coli. We aim at efficiently converting renewable and abundant natural resources into higher value products using microbes (especially E. coli). Combinatorial (evolutionary) techniques are mainly used to engineer the biosynthesis pathways to improve the product yields or pathway efficiencies.
9/2003-2/2006 Scholarship of Korea Science and Engineering Foundation

1.Yanyan Lei, Wei Chen, La Xiang, Jieyuan Wu, Zhen Zhen, Jian-Ming Jin*, Chaoning Liang*, Shuang-Yan Tang*. Engineering an SspB-mediated degron for novel controllable protein degradation. Metabolic Engineering 2022, 74: 150-159. 

2.Xuanxuan Zhang#, Yang He#, Zhe Wu, Guoxia Liu, Yong Tao, Jian-Ming Jin*, Wei Chen*, Shuang-Yan Tang*. Whole-cell biosensors aid exploration of vanillin transmembrane transport. Journal of Agricultural and Food Chemistry 2021, 69 (10): 3114-3123.

3.Shizhong Li#, Chaoning Liang#, Guoxia Liu, Jian-Ming Jin*, Yong Tao*, Shuang-Yan Tang*. De novo biosynthesis of chlorogenic acid using an artificial microbial community. Journal of Agricultural and Food Chemistry 2021, 69 (9): 2816-2825.

4.Jieyuan Wu, Wei Chen, Yutong Zhang, Xuanxuan Zhang, Jian-Ming Jin*, Shuang-Yan Tang*. Metabolic engineering for improved curcumin biosynthesis in Escherichia coli. Journal of Agricultural and Food Chemistry 2020, 68 (39):10772-10779

5.Xiangyu Ji, Huiwei Zhao, Hua Zhu, Kun Zhu*, Shuang-Yan Tang*, Chunbo Lou*. CRISPRi/dCpf1-mediated dynamic metabolic switch to enhance butenoic acid production in Escherichia coli. Applied Microbiology and Biotechnology 2020, 104: 5385-5393.

6. Jun Yao, Yang He, Nannan Su, Sakshibeedu R. Bharath, Yong Tao, Jian-Ming Jin*, Wei Chen*, Haiwei Song*, Shuang-Yan Tang*. Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps. Nature Communications 2020, 11: 1515.

7. Jun Yao#, Jing Li#, Dandan Xiong, Yuanyuan Qiu, Guizhi Shi, Jian-Ming Jin*, Yong Tao*, Shuang-Yan Tang*. Development of a highly efficient and specific l-theanine synthase. Applied Microbiology and Biotechnology 2020, 

8. Chaoning Liang, Xuanxuan Zhang, Jieyuan Wu, Shanshan Mu, Zhe Wu, Jian-Ming Jin*, Shuang-Yan Tang*, Dynamic control of toxic natural product biosynthesis by an artificial regulatory circuit. Metabolic Engineering 2020, 57: 239-246 

9. Wei Chen#, Jun Yao#, Jie Meng, Wenjing Han, Yong Tao, Yihua Chen, Yixin Guo, Guizhi Shi, Yang He*, Jian-Ming Jin*, Shuang-Yan Tang*, Promiscuous enzymatic activity-aided multiple-pathway network design for metabolic flux rearrangement in hydroxytyrosol biosynthesis. Nature Communications 2019, 10: 960. 

10. Wei Chen#, Xuanxuan Zhang#, Dandan Xiong#, Jian-Ming Jin*, Shuang-Yan Tang*. Engineering the effector specificity of regulatory proteins for the in vitro detection of biomarkers and pesticide residues. Applied Microbiology and Biotechnology 2019, 103: 3205-3213. 

11. Heng Li, Jing Li, Ruinan Jin, Wei Chen, Chaoning Liang, Jieyuan Wu, Jian-Ming Jin, Shuang-Yan Tang*, Towards the construction of high-quality mutagenesis libraries. Biotechnology Letters 2018, 40: 1101-1107. 

12. Heng Li, Wei Chen, Ruinan Jin, Jian-Ming Jin*, Shuang-Yan Tang*, Biosensor-aided high-throughput screening of hyper-producing cells for malonyl-CoA-derived products. Microbial Cell Factories 2017, 16: 187. 

13. Heng Li, Chaoning Liang, Wei Chen, Jian-Ming Jin*, Shuang-Yan Tang*, Yong Tao, Monitoring in vivo metabolic flux with a designed whole-cell metabolite biosensor of shikimic acid. Biosensors and Bioelectronics 2017, 98: 457-465.  

14. Jieyuan Wu, Peixia Jiang, Wei Chen, Dandan Xiong, Linglan Huang, Junying Jia, Yuanyuan Chen, Jian-Ming Jin*, Shuang-Yan Tang*, Design and application of a lactulose biosensor. Scientific Reports 2017, 7: 45994. 

15. Qingzhuo Wang, Shuang-Yan Tang*, Sheng Yang*. Genetic biosensors for small-molecule products: Design and applications in high-throughput screening. Frontiers of Chemical Science and Engineering 2017, 11: 15-26.

16. Dandan Xiong, Shikun Lu, Jieyuan Wu, Chaoning Liang, Wei Wang, Wenzhao Wang, Jian-Ming Jin*, Shuang-Yan Tang*, Improving key enzyme activity in phenylpropanoid pathway with a designed biosensor. Metabolic Engineering 2017, 40: 115-123. 

17. Lin Zhu#, Zhe Wu#, Jian-Ming Jin, Shuang-Yan Tang*, Directed evolution of leucine dehydrogenase for improved efficiency of l-tert-leucine synthesis. Applied Microbiology and Biotechnology 2016, 100: 5805-5813. 

18. Chaoning Liang#, Yi Zhang#, Yan Jia, Wenzhao Wang, Youhai Li, Shikun Lu, Jian-Ming Jin*, Shuang-Yan Tang*. Engineering a carbohydrate-processing transglycosidase into glycosyltransferase for natural product glycodiversification. Scientific Reports 2016, 6: 21051.  

19. Wei Chen#, Shan Zhang#, Peixia Jiang#, Jun Yao, Yongzhi He, Lincai Chen, Xiwu Gui, Zhiyang Dong*, Shuang-Yan Tang*. Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis. Metabolic Engineering 2015, 30:149-155. 

20. Peixia Jiang#, Shanshan Mu#, Heng Li, Youhai Li, Congmin Feng, Jian-Ming Jin*, Shuang-Yan Tang*. Design and application of a novel high-throughput screening technique for 1-deoxynojirimycin. Scientific Reports 2015, 5: 8563. 

21. Chaoning Liang#, Xiwu Gui#, Cheng Zhou, Yanfen Xue, Yanhe Ma*, Shuang-Yan Tang*. Improving the thermoactivity and thermostability of pectate lyase from Bacillus pumilus for ramie degumming. Applied Microbiology and Biotechnology 2015, 99: 2673-2682. 

22. Chaoning Liang#, Dandan Xiong#, Yi Zhang, Shanshan Mu, Shuang-Yan Tang*. Development of a novel uric-acid-responsive regulatory system in Escherichia coli. Applied Microbiology and Biotechnology 2015, 99:2267-2275. 

23. Shuang-Yan Tang, Shuai Qian, Olubolaji Akinterinwa, Christopher S. Frei, Joseph A. Gredell, Patrick C. Cirino. Screening for enhanced triacetic acid lactone production by recombinant Escherichia coli expressing a designed triacetic acid lactone reporter. Journal of the American Chemical Society 2013, 135 (27): 10099-10103.  

24. Shuang-Yan Tang, Patrick C. Cirino. Design and application of a mevalonate- responsive regulatory protein. Angewandte Chemie International Edition 2011, 50: 1084-1086. 

25. Shuang-Yan Tang, Patrick C. Cirino. Elucidating residue roles in engineered variants of AraC regulatory protein. Protein Science 2010, 19: 291-298. 

26. Shuang-Yan Tang, Hossein Fazelinia, Patrick C. Cirino. AraC regulatory protein   mutants with altered effector specificity. Journal of the American Chemical Society  2008, 130: 5267-5271.  

27. Shuang-Yan Tang, Sung-Jae Yang, Hyunju Cha, Eui-Jeon Woo, Cheonseok Park, Kwan-Hwa Park. Contribution of W229 to the transglycosylation activity of 4-α- glucanotransferase from Pyrococcus furiosus. Biochimica et Biophysica Acta 2006, 1764: 1633-1638.  

28. Shuang-Yan Tang, Quang-Tri Le, Jae-Hoon Shim, Sung-Jae Yang, Joong-Huck Auh,  Cheonseok Park, Kwan-Hwa Park. Enhancing thermostability of maltogenic amylase from Bacillus thermoalkalophilus ET2 by DNA shuffling. FEBS Journal, 2006, 273: 3335-3345.

29. Kyung-Ah Cheong,# Shuang-Yan Tang, # Tae-Kyu Cheong, Hyunju Cha, Jung-Wan Kim, Kwan-Hwa Park. Thermostable and alkalophilic maltogenic amylase of Bacillus thermoalkalophilus ET2 in monomer-dimer equilibrium. Biocatalysis and Biotransformation, 2005, 23: 79-/87  # Contributed equally 

30. Dan Li, Sung-Hoon Park, Jae-Hoon Shim, Hee-Seob Lee, Shuang-Yan Tang, Cheon- Seok Park and Kwan-Hwa Park. In vitro enzymatic modification of puerarin to puerarin glycosides by maltogenic amylase. Carbohydrate Research 2004, 339: 2789-2797.  

31. 李恒, 唐双焱*. 碳水化合物结合结构域研究进展[J]. 微生物学报, 2017, 57(8): 1160-1167. 

32. 唐双焱*梁朝宁,蒋培霞. 高通量筛选工具的设计与应用 生物工程学报,2012, 28(7): 781-788.